N-Cadherin antibody (AA 308-597)

Catalog No. ABIN7873738

The Rat Monoclonal anti-N-Cadherin antibody (Clone MNCD2) (ABIN7873738) specifically detects N-Cadherin in IHC (p). The antibody is reactive with Human and Mouse samples.

Quick Overview for N-Cadherin antibody (AA 308-597) (ABIN7873738)

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Reactivity: Human, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This N-Cadherin antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: MNCD2

Product Details anti-N-Cadherin Antibody

Binding Specificity: AA 308-597

Purpose: CD325 Antibody / CDH2 / N-Cadherin

Purification: Protein A/G affinity

Immunogen: A portion of amino acids 308-597 was used as the immunogen for the CD325 antibody.

Isotype: IgG2a

Application Details

Application Notes: Optimal dilution of the CD325 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the CD325 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for N-Cadherin

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Alternative Name: CD325

Background: Recognizes a protein of ~140 kDa, identified as N-Cadherin (NCAD), also known as CD325. NCAD is a member of the Cadherin superfamily, and consists of five extracellular repeats, a transmembrane domain and a cytoplasmic domain. CD325 deficient mice die at day 10 of gestation and embryos display major heart defects and malformed neural tubes and somites. Consistent with this, CD325 has been implicated in several aspects of cardiac development including the precardiac mesoderm, establishment of left-right symmetry and cardiac looping morphogenesis. Furthermore, CD325 is normally involved in inducing cell cycle arrest and its expression is frequently deregulated in cancer cells.Studies have linked N-cadherin to cancer metastasis by showing the aggressive tumor cells had preferentially turned on N-cadherin as opposed to E- or P-cadherin.

UniProt: P19022

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane