SAP130 antibody (AA 309-913)

Catalog No. ABIN7873743

The Rabbit Polyclonal anti-SAP130 antibody is suitable to detect SAP130 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC (p), IF and FACS.

Quick Overview for SAP130 antibody (AA 309-913) (ABIN7873743)

Target: SAP130 (Sin3A-Associated Protein, 130kDa (SAP130))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SAP130 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-SAP130 Antibody

Binding Specificity: AA 309-913

Purpose: SAP130 Antibody / Sin3-associated polypeptide p130

Purification: Antigen affinity purified

Immunogen: E. coli-derived recombinant human protein (amino acids H309-H913) was used as the immunogen for the SAP130 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SAP130 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SAP130 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for SAP130

Target: SAP130 (Sin3A-Associated Protein, 130kDa (SAP130))

Alternative Name: SAP130

Background: Histone deacetylase complex subunit SAP130 is an enzyme that in humans is encoded by the SAP130 gene. This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair.

UniProt: Q9H0E3