SNAP25 antibody (AA 31-203)

Catalog No. ABIN7873753

The Rabbit Polyclonal anti-SNAP25 antibody (ABIN7873753) specifically detects SNAP25 in WB, ELISA and FACS. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for SNAP25 antibody (AA 31-203) (ABIN7873753)

Target: SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SNAP25 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-SNAP25 Antibody

Binding Specificity: AA 31-203

Purpose: SNAP25 Antibody / Synaptosomal-associated protein 25

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human SNAP25 recombinant protein (Position: R31-L203) was used as the immunogen for the SNAP25 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SNAP25 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SNAP25 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for SNAP25

Target: SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Alternative Name: SNAP25

Background: SNAP25 antibody detects Synaptosomal-associated protein 25, a key component of the SNARE complex responsible for synaptic vesicle docking and neurotransmitter release. SNAP25 is essential for calcium-triggered exocytosis in neurons and neuroendocrine cells, making it a crucial mediator of synaptic transmission. The SNAP25 antibody is widely used in neurobiology to study vesicle fusion, neurotransmission, and synaptic plasticity.

SNAP25 is encoded by the SNAP25 gene located on human chromosome 20p12.2. The protein is approximately 25 kilodaltons and anchored to the plasma membrane via palmitoylated cysteine residues in its central linker region. SNAP25 interacts with syntaxin and synaptobrevin (VAMP) to form the ternary SNARE complex, which brings vesicle and plasma membranes into close proximity for fusion. This complex enables rapid and precise neurotransmitter release at presynaptic terminals.

The SNAP25 antibody detects a 25 kilodalton band by western blot and shows punctate presynaptic staining in neurons and neuroendocrine cells. SNAP25 participates in multiple exocytic events, including insulin secretion, catecholamine release, and synaptic vesicle recycling. It also regulates short-term synaptic plasticity by modulating vesicle pool dynamics.

Phosphorylation of SNAP25 by protein kinases such as PKC and CDK5 fine-tunes its role in vesicle priming and fusion probability. Mutations or altered expression of SNAP25 are linked to neurological and psychiatric disorders, including attention deficit hyperactivity disorder, epilepsy, and schizophrenia. Reduced SNAP25 function leads to synaptic transmission defects and cognitive impairment.

In addition to neurons, SNAP25 expression occurs in pancreatic beta-cells and endocrine tissues, reflecting its conserved role in vesicle exocytosis. NSJ Bioreagents provides a validated SNAP25 antibody optimized for western blot, flow cytometry, and neuronal culture staining, supporting detailed analysis of neurotransmitter release, synaptic machinery, and neurosecretory regulation.

UniProt: P60880

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport