UBR5 antibody (AA 31-761)

Catalog No. ABIN7873803

The Rabbit Polyclonal anti-UBR5 antibody is suitable to detect UBR5 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and FACS.

Quick Overview for UBR5 antibody (AA 31-761) (ABIN7873803)

Target: UBR5 (Ubiquitin Protein Ligase E3 Component N-Recognin 5 (UBR5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBR5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-UBR5 Antibody

Binding Specificity: AA 31-761

Purpose: UBR5 Antibody / hHYD

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human UBR5 recombinant protein (Position: K31-K761) was used as the immunogen for the UBR5 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the UBR5 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the UBR5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for UBR5

Target: UBR5 (Ubiquitin Protein Ligase E3 Component N-Recognin 5 (UBR5))

Alternative Name: UBR5

Background: UBR5 antibody detects E3 ubiquitin-protein ligase UBR5, a large multidomain enzyme that catalyzes ubiquitination of specific substrate proteins, regulating DNA damage repair, transcription, and cell cycle control. UBR5 belongs to the HECT-type E3 ligase family and plays a critical role in maintaining genomic integrity and proteostasis. The UBR5 antibody is widely used in studies of ubiquitin signaling, cancer biology, and DNA repair mechanisms.

UBR5 is encoded by the UBR5 gene located on human chromosome 8q22.3. The protein is approximately 300 kilodaltons and contains a C-terminal HECT domain responsible for ubiquitin transfer, an N-terminal UBR box involved in substrate recognition, and several WW domains that mediate protein-protein interactions. UBR5 localizes to both the nucleus and cytoplasm, functioning in proteasomal degradation and signaling complex assembly.

The UBR5 antibody detects a 300 kilodalton band in western blot analysis and shows both nuclear and cytoplasmic staining under immunofluorescence. UBR5 ubiquitinates regulators of the DNA damage response such as BRCA1 and p53, coordinating checkpoint activation and repair. It also targets transcription factors and signaling intermediates, integrating stress responses with transcriptional control.

UBR5 is essential for embryonic development and cell viability. Its dysregulation is associated with multiple cancers, including breast, ovarian, and colorectal carcinoma, where it functions as either an oncogene or tumor suppressor depending on context. Overexpression enhances proliferation and metastasis, while depletion sensitizes cells to DNA-damaging agents and promotes apoptosis.

As a master regulator of ubiquitin signaling, UBR5 links proteolytic control with cell fate decisions. NSJ Bioreagents provides a validated UBR5 antibody optimized for western blot and flow cytometry, supporting research into ubiquitination pathways, DNA damage responses, and oncogenic signaling networks.

UniProt: O95071

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway