SIL1 antibody (AA 32-448)

Catalog No. ABIN7873923

The Rabbit Polyclonal anti-SIL1 antibody is suitable to detect SIL1 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC, FACS and IF.

Quick Overview for SIL1 antibody (AA 32-448) (ABIN7873923)

Target: SIL1 (Nucleotide Exchange Factor SIL1 (SIL1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIL1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-SIL1 Antibody

Binding Specificity: AA 32-448

Purpose: SIL1 Antibody / Nucleotide exchange factor SIL1 / BiP-associated protein

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human SIL1 recombinant protein (Position: H32-E448) was used as the immunogen for the SIL1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SIL1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SIL1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for SIL1

Target: SIL1 (Nucleotide Exchange Factor SIL1 (SIL1))

Alternative Name: SIL1

Background: SIL1 antibody detects Nucleotide exchange factor SIL1, encoded by the SIL1 gene. Nucleotide exchange factor SIL1 is an endoplasmic reticulum-localized protein that functions as a co-chaperone for the HSPA5/BiP chaperone, facilitating nucleotide exchange and proper folding of secretory and membrane proteins. SIL1 antibody provides researchers with a specialized reagent for studying ER protein homeostasis, unfolded protein response signaling, and genetic diseases associated with ER stress.

Nucleotide exchange factor SIL1 is part of the endoplasmic reticulum protein quality control machinery. Research using SIL1 antibody has shown that it interacts with HSPA5 to catalyze the release of ADP from the chaperone, allowing ATP binding and restarting of the folding cycle. This nucleotide exchange activity is crucial for maintaining an efficient cycle of protein folding and preventing accumulation of misfolded proteins in the ER.

Studies with SIL1 antibody have revealed that mutations in SIL1 cause Marinesco-Sji 1/2gren syndrome, a rare multisystem disorder characterized by cerebellar ataxia, congenital cataracts, muscle weakness, and intellectual disability. These findings demonstrate the essential role of SIL1 in maintaining ER homeostasis and proteostasis in multiple tissues. In model organisms, loss of SIL1 disrupts protein folding, leading to widespread ER stress and cellular dysfunction.

Dysregulation of Nucleotide exchange factor SIL1 has also been implicated in neurodegeneration and metabolic disease. Research using SIL1 antibody has shown that altered expression contributes to vulnerability of neurons and muscle cells to ER stress, while overexpression in certain contexts can be protective. These dual roles emphasize the importance of tightly regulated SIL1 activity in health and disease.

SIL1 antibody is widely applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting quantifies expression in tissues and cell lines, immunohistochemistry demonstrates localization in pancreas, muscle, and brain, and immunofluorescence highlights ER localization. These applications make SIL1 antibody indispensable for ER stress and proteostasis research.

By supplying validated SIL1 antibody reagents, NSJ Bioreagents supports studies into ER chaperone networks, protein misfolding diseases, and stress responses. Detection of Nucleotide exchange factor SIL1 provides researchers with insight into how ER co-chaperones regulate folding efficiency and human disease.

UniProt: Q9H173

Pathways: Unfolded Protein Response, SARS-CoV-2 Protein Interactome