PTK2B antibody (AA 35-770)

Catalog No. ABIN7874179

The Rabbit Polyclonal anti-PTK2B antibody (ABIN7874179) specifically detects PTK2B in WB, IHC, ELISA, IF, ICC and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for PTK2B antibody (AA 35-770) (ABIN7874179)

Target: PTK2B (PTK2B Protein tyrosine Kinase 2 beta (PTK2B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PTK2B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-PTK2B Antibody

Binding Specificity: AA 35-770

Purpose: PTK2B Antibody / PYK2 / Protein tyrosine kinase 2 beta

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human PYK2/PTK2B recombinant protein (Position: K35-R770) was used as the immunogen for the PTK2B antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PTK2B antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the PTK2B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PTK2B

Target: PTK2B (PTK2B Protein tyrosine Kinase 2 beta (PTK2B))

Alternative Name: PTK2B

Background: PTK2B antibody detects Protein tyrosine kinase 2 beta, also known as PYK2, a non-receptor tyrosine kinase involved in calcium-dependent signal transduction, cytoskeletal remodeling, and synaptic plasticity. Encoded by the PTK2B gene on chromosome 8p21.2, PYK2 belongs to the focal adhesion kinase (FAK) family and mediates signals downstream of G-protein-coupled receptors, integrins, and ion channels. It is abundantly expressed in brain, immune cells, and epithelial tissues, where it regulates adhesion, migration, and intracellular calcium responses.

Structurally, PYK2 is a 1,009-amino-acid cytoplasmic protein of approximately 116 kilodaltons containing an N-terminal FERM domain for membrane association, a central kinase domain, and a C-terminal focal adhesion-targeting (FAT) domain. Activation occurs through autophosphorylation at tyrosine 402, creating a binding site for Src family kinases and downstream effectors. The protein localizes to focal adhesions and postsynaptic densities, linking calcium signals to cytoskeletal dynamics and synaptic strength.

The PTK2B antibody is widely used in neuroscience, immunology, and cancer biology research to study focal adhesion signaling, neuronal activity, and cell migration. Western blot analysis detects a 116 kilodalton band corresponding to PYK2, while immunofluorescence shows punctate cytoplasmic and perinuclear staining consistent with activated signaling foci. This antibody enables exploration of calcium-sensitive kinase pathways and their contribution to cell communication and motility.

Functionally, PYK2 mediates integrin and calcium-dependent signaling that regulates cytoskeletal rearrangement, vesicle trafficking, and neuronal transmission. In the nervous system, PYK2 modulates synaptic plasticity and learning, while in immune cells it controls adhesion and migration. Dysregulation of PTK2B expression or activity has been linked to Alzheimer's disease, inflammatory disorders, and cancer metastasis. The PTK2B antibody provides a versatile reagent for investigating kinase activation, intracellular signaling dynamics, and calcium-dependent regulation. NSJ Bioreagents validates this antibody for its applications, ensuring specificity and reproducibility in studies of kinase signaling and cellular motility.

UniProt: Q14289

Pathways: EGFR Signaling Pathway, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Cell-Cell Junction Organization, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGF Signaling