MSH6 antibody (AA 374-540)

Catalog No. ABIN7874451

The Mouse Monoclonal anti-MSH6 antibody is suitable to detect MSH6 in samples from Human. It has been validated for IHC (p).

Quick Overview for MSH6 antibody (AA 374-540) (ABIN7874451)

Target: MSH6 (MutS Homolog 6 (E. Coli) (MSH6))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MSH6 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: MST6-2

Product Details anti-MSH6 Antibody

Binding Specificity: AA 374-540

Purpose: MSH6 Antibody

Purification: Protein G affinity chromatography

Immunogen: A recombinant human MSH6 protein fragment within amino acids 374-540 was used as the immunogen for the MSH6 antibody.

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal dilution of the MSH6 antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the MSH6 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for MSH6

Target: MSH6 (MutS Homolog 6 (E. Coli) (MSH6))

Alternative Name: MSH6

Background: MSH6 is a component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. [UniProt]

UniProt: P52701

Pathways: DNA Damage Repair, Chromatin Binding, Production of Molecular Mediator of Immune Response