MSH6 antibody (AA 374-540)

Catalog No. ABIN7874454

The Mouse Monoclonal anti-MSH6 antibody is suitable to detect MSH6 in samples from Human. It has been validated for IHC (p).

Quick Overview for MSH6 antibody (AA 374-540) (ABIN7874454)

Target: MSH6 (MutS Homolog 6 (E. Coli) (MSH6))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MSH6 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: MSH6-3086

Product Details anti-MSH6 Antibody

Binding Specificity: AA 374-540

Purpose: MSH6 Antibody

Purification: Protein G affinity chromatography

Immunogen: A recombinant human MSH6 protein fragment within amino acids 374-540 was used as the immunogen for the MSH6 antibody.

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal dilution of the MSH6 antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the MSH6 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for MSH6

Target: MSH6 (MutS Homolog 6 (E. Coli) (MSH6))

Alternative Name: MSH6

Background: The finding that mutations in DNA mismatch repair genes are associated with hereditary nonpolyposis colorectal cancer (HNPCC) has resulted in considerable interest in the understanding of the mechanism of DNA mismatch repair. Initially, inherited mutations in the MSH2 and MLH1 homologs of the bacterial DNA mismatch repair genes mutS and mutL were demonstrated at high frequency in HNPCC and were shown to be associated with microsatellite instability. A member of the mismatch repair family, GTBP (also designated MSH6), is an MSH2-related protein that binds to DNA containing G/T mismatches. Findings suggest that the mismatch-binding factor in human cells is composed of a heterodimer of GTBP and MSH2.

UniProt: P52701

Pathways: DNA Damage Repair, Chromatin Binding, Production of Molecular Mediator of Immune Response