Replication Termination Factor 2 (RTF2) (AA 4-306) antibody

Catalog No. ABIN7874668

The Rabbit Polyclonal anti-Replication Termination Factor 2 antibody (ABIN7874668) specifically detects Replication Termination Factor 2 in WB, ELISA, IP, FACS, ICC and IF. The antibody is reactive with Human samples.

Quick Overview for Replication Termination Factor 2 (RTF2) (AA 4-306) antibody (ABIN7874668)

Target: Replication Termination Factor 2 (RTF2)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details

Binding Specificity: AA 4-306

Purpose: RTF2 Antibody / Replication termination factor 2

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human RTF2 recombinant protein (Position: D4-F306) was used as the immunogen for the RTF2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the RTF2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the RTF2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details

Target: Replication Termination Factor 2 (RTF2)

Alternative Name: RTF2

Background: The RTF2 antibody targets Replication termination factor 2, a nuclear protein encoded by the RTF2 gene that functions in DNA replication and replication-fork stability. Replication termination factor 2 is a component of the replication-stress response pathway that safeguards genome integrity by promoting proper fork processing and restart. The RTF2 antibody provides an essential tool for studying DNA replication, repair mechanisms, and cell-cycle regulation.

Replication termination factor 2 associates with replication forks and interacts with the replisome to stabilize stalled replication structures. It is recruited to chromatin during S phase and removed after replication completion. The RTF2 antibody supports visualization of these dynamics, allowing researchers to monitor fork progression and resolution under genotoxic stress. RTF2 is critical for preventing aberrant fork degradation and double-strand break formation, thereby maintaining genomic stability.

Loss of RTF2 leads to replication defects, increased DNA damage signaling, and activation of checkpoint kinases such as ATR and CHK1. The RTF2 antibody enables detection of expression changes in response to DNA damage or replication inhibitors, aiding studies into checkpoint control and genome maintenance. Its depletion causes hypersensitivity to replication-blocking agents and reduced cell viability, emphasizing its protective role during S phase.

Replication termination factor 2 also participates in fork restart after hydroxyurea-induced stalling, coordinating reloading of replication machinery components. The RTF2 antibody allows mechanistic studies of this recovery process, revealing how RTF2 collaborates with helicases and nucleases to reestablish replication competence. Dysregulation of RTF2 expression has been implicated in cancer development and progression due to impaired replication fidelity.

The RTF2 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, showing characteristic nuclear staining consistent with its role in DNA metabolism. NSJ Bioreagents provides this antibody with validated specificity and reproducibility for use in molecular-genetics, oncology, and replication research. By supporting detailed analysis of Replication termination factor 2, the RTF2 antibody advances understanding of DNA replication dynamics, fork stability, and genome-maintenance pathways.

UniProt: Q9BY42