SPRED2 antibody (AA 40-298)

Catalog No. ABIN7874694

The Rabbit Polyclonal anti-SPRED2 antibody is suitable to detect SPRED2 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC (p), IF and FACS.

Quick Overview for SPRED2 antibody (AA 40-298) (ABIN7874694)

Target: SPRED2 (Sprouty-Related, EVH1 Domain Containing 2 (SPRED2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPRED2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-SPRED2 Antibody

Binding Specificity: AA 40-298

Purpose: SPRED2 Antibody / Sprouty-related EVH1 domain-containing protein 2

Purification: Antigen affinity purified

Immunogen: Amino acids R40-R298 from the human protein were used as the immunogen for the SPRED2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SPRED2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SPRED2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for SPRED2

Target: SPRED2 (Sprouty-Related, EVH1 Domain Containing 2 (SPRED2))

Alternative Name: SPRED2

Background: SPRED2 (Sprouty-related EVH1 domain-containing protein 2) is a member of the SPRED protein family, which negatively regulates receptor tyrosine kinase (RTK) signaling pathways. By interacting with components of the Ras/MAPK cascade, SPRED2 suppresses excessive ERK activation, thereby controlling cellular processes such as proliferation, differentiation, and migration. Researchers frequently use a SPRED2 antibody to investigate signaling dynamics and the molecular mechanisms of growth factor regulation.

SPRED2 contains an N-terminal Ena/VASP homology 1 (EVH1) domain, a central c-Kit binding domain, and a C-terminal Sprouty-related cysteine-rich domain. These structural motifs allow SPRED2 to localize to the plasma membrane and recruit signaling proteins, where it inhibits Ras and Raf activation. Employing a SPRED2 antibody allows scientists to study how this protein maintains signaling balance across a variety of cell types.

Altered expression of SPRED2 has been linked to human disease. Loss or reduction of SPRED2 function has been associated with enhanced MAPK pathway activity, which may contribute to oncogenesis and abnormal tissue growth. Additionally, SPRED2 is studied in the context of immune regulation and inflammation, where dysregulated signaling can lead to pathological outcomes. Using a SPRED2 antibody supports research into these processes and offers insight into therapeutic strategies aimed at modulating Ras/MAPK signaling.

NSJ Bioreagents offers a high-quality SPRED2 antibody validated for applications such as western blot, immunohistochemistry, and immunofluorescence. Choosing a SPRED2 antibody from NSJ Bioreagents ensures reliable detection and reproducible performance in studies of signal transduction, cell regulation, and disease biology.

UniProt: Q7Z698