Recombinant SMAD4 antibody (AA 400-552)

Catalog No. ABIN7874725

The Rabbit Monoclonal anti-SMAD4 antibody (Clone SMAD4-7906R) (ABIN7874725) specifically detects SMAD4 in IHC (p). The antibody is reactive with Human samples.

Quick Overview for Recombinant SMAD4 antibody (AA 400-552) (ABIN7874725)

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SMAD4 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SMAD4-7906R

Product Details anti-SMAD4 Antibody

Binding Specificity: AA 400-552

Purpose: Recombinant SMAD4 Antibody

Purification: Protein A/G affinity

Immunogen: A recombinant partial protein sequence (within amino acids 400-552) from the human protein was used as the immunogen for the recombinant SMAD4 antibody.

Isotype: IgG, kappa

Application Details

Application Notes: Optimal dilution of the recombinant SMAD4 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot the recombinant SMAD4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for SMAD4

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Alternative Name: SMAD4

Background: Signaling from the ligand-activated membrane receptor serine/threonine kinases to nuclear targets is mediated by a set of evolutionarily conserved proteins known as DPC4. Upon ligand binding, the receptors of the TGF-beta family phosphorylate SMAD proteins (SMAD1 and SMAD2). These proteins then move into the nucleus, where they activate transcription. To carry out this function, the receptor activated SMAD1 and 2 require association with the product of deleted in pancreatic carcinoma, locus 4 (DPC4), also known as SMAD4. SMAD4/DPC4 is also implicated as a tumor suppressor, since it is inactivated in more than half of pancreatic carcinomas and to a lesser extent in a variety of other cancers. The lack of SMAD4 expression is present in approximately 80 % of cases of pancreatic adenocarcinoma, but rarely in endometrial (0 %), colorectal (0 %), ovarian (3 %), lung (0 %), breast (2 %) adenocarcinomas, and malignant melanoma (4 %). SMAD4is an important marker for confirming a diagnosis of pancreatic adenocarcinoma.

UniProt: Q13485

Pathways: Cell Division Cycle, Chromatin Binding, Autophagy