MSK1 antibody (pSer376)

Catalog No. ABIN744008

The Rabbit Polyclonal anti-MSK1 antibody has been validated for WB, ELISA, IF (cc), IF (p), IHC (p) and IHC (fro). It is suitable to detect MSK1 in samples from Mouse.

Quick Overview for MSK1 antibody (pSer376) (ABIN744008)

Target: MSK1 (RPS6KA5) (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 5 (RPS6KA5))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MSK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-MSK1 Antibody

Binding Specificity: pSer376

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Pig,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human MSK1 around the phosphorylation site of Ser376 [GY(p-S)FV]

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for MSK1

Target: MSK1 (RPS6KA5) (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 5 (RPS6KA5))

Alternative Name: MSK1

Background:

Synonyms: MSK1 Phospho Ser376, 90 kDa ribosomal protein S6 kinase 5, EC 2.7.11.1, KS6A5_HUMAN, MGC1911, Mitogen and stress activated protein kinase 1, MSPK1, Nuclear Mitogen And Stress Activated Protein Kinase 1, Nuclear mitogen- and stress-activated protein kinase 1, Ribosomal protein S6 kinase 90kD polypeptide 5, Ribosomal protein S6 kinase 90 kDa, Ribosomal protein S6 kinase 90 kDa polypeptide 5, Ribosomal Protein S6 Kinase Alpha 5, Ribosomal protein S6 kinase alpha-5, RLPK, RPS6KA5, RSK Like Protein Kinase, RSK-like protein kinase, RSKL, S6K alpha 5, S6K-alpha-5.

Background: MSK1 is a mitogen and stress activated protein kinase 1 which belongs to the AGC family of kinases and is related in structure to the ribosomal p70 S6 kinase subfamily. MSK1 can be activated by ERK1/2 and SAPK2/p38 MAP kinase. It is also known to be required for the phosphorylation of CREB, ATF1 H3 and HMG14 in response to mitogen and stress. Similar to RSK, MSK1 contains two kinase domains (N term and a C term). Once phosphorylated on Thr581 and Ser360 by ERK1/2 and SAPK2/p38, MSK1 autophosphorylate on at least 5 sites. Of these autophosphorylation sites Ser212 and Ser376 get phosphorylated by the C terminal kinase domain of MSK1 which is essential for the catalytic activity of the N terminal kinase domain.

Gene ID: 9252

UniProt: O75582

Pathways: MAPK Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades