SOAT1 antibody (AA 41-548)

Catalog No. ABIN7874850

The Rabbit Polyclonal anti-SOAT1 antibody is suitable to detect SOAT1 in samples from Human, Rat and Mouse. It has been validated for WB, ELISA, IHC, IF, ICC and FACS.

Quick Overview for SOAT1 antibody (AA 41-548) (ABIN7874850)

Target: SOAT1 (Sterol O-Acyltransferase 1 (SOAT1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SOAT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-SOAT1 Antibody

Binding Specificity: AA 41-548

Purpose: SOAT1 Antibody / Sterol O-acyltransferase 1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human SOAT1 recombinant protein (Position: R41-Y548) was used as the immunogen for the SOAT1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SOAT1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SOAT1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for SOAT1

Target: SOAT1 (Sterol O-Acyltransferase 1 (SOAT1))

Alternative Name: SOAT1

Background: SOAT1 antibody detects Sterol O-acyltransferase 1, an integral endoplasmic reticulum enzyme responsible for the esterification of cholesterol to form cholesterol esters. The UniProt recommended name is Sterol O-acyltransferase 1 (SOAT1), also known as Acyl-CoA:cholesterol acyltransferase 1 (ACAT1). This enzyme plays a central role in intracellular cholesterol homeostasis, lipid storage, and steroidogenesis.

Functionally, SOAT1 antibody identifies a 550-amino-acid multi-pass membrane enzyme that catalyzes the formation of cholesterol esters by transferring fatty acyl groups from acyl-CoA to the hydroxyl group of cholesterol. These neutral esters are stored in cytoplasmic lipid droplets, buffering free cholesterol concentrations within membranes. SOAT1 activity is critical for maintaining cellular lipid balance and protecting against cholesterol-induced cytotoxicity.

The SOAT1 gene is located on chromosome 1q25.2 and encodes a protein embedded in the endoplasmic reticulum membrane. It operates as a key metabolic switch regulating cholesterol storage and membrane composition. In macrophages, SOAT1 converts excess cholesterol into cholesteryl esters, preventing foam cell formation and atherogenesis. In steroidogenic tissues, it contributes to the availability of cholesterol precursors for hormone synthesis. Dysregulation of SOAT1 disrupts lipid homeostasis and is implicated in metabolic disorders, neurodegeneration, and cardiovascular disease.

SOAT1 functions alongside SOAT2, which is primarily expressed in the liver and intestine, while SOAT1 is ubiquitously expressed in peripheral tissues. Its activity is regulated by intracellular cholesterol levels, oxysterols, and acyl-CoA availability. Pharmacological inhibition of SOAT1 has been explored as a strategy to reduce atherosclerotic plaque formation and neurotoxic cholesterol accumulation in Alzheimer's disease.

SOAT1 antibody is widely used in lipid metabolism, cardiovascular, and neurobiology research. It is suitable for immunoblotting, immunofluorescence, and enzyme localization studies to evaluate intracellular cholesterol esterification. This antibody enables the investigation of lipid droplet formation, cholesterol trafficking, and sterol regulatory pathways. In metabolic studies, SOAT1 serves as a marker of intracellular cholesterol storage and lipid imbalance.

Structurally, SOAT1 is an endoplasmic reticulum membrane protein containing multiple transmembrane domains and conserved histidine residues essential for catalytic function. The enzyme operates as a homotetramer, with each subunit contributing to the catalytic center. NSJ Bioreagents provides SOAT1 antibody reagents validated for use in lipid metabolism, cholesterol transport, and metabolic disease research.

UniProt: P35610