BTBD12 antibody (AA 42-1804)

Catalog No. ABIN7874912

This Rabbit Polyclonal antibody specifically detects BTBD12 in IHC, ELISA, IF and ICC. It exhibits reactivity toward Human.

Quick Overview for BTBD12 antibody (AA 42-1804) (ABIN7874912)

Target: BTBD12 (BTB (POZ) Domain Containing 12 (BTBD12))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BTBD12 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-BTBD12 Antibody

Binding Specificity: AA 42-1804

Purpose: SLX4 Antibody / Structure-specific endonuclease subunit SLX4

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human SLX4 recombinant protein (Position: Q42-H1804) was used as the immunogen for the SLX4 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SLX4 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SLX4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for BTBD12

Target: BTBD12 (BTB (POZ) Domain Containing 12 (BTBD12))

Alternative Name: SLX4

Background: The SLX4 antibody targets Structure-specific endonuclease subunit SLX4, a multifunctional DNA repair scaffold protein encoded by the SLX4 gene. SLX4 coordinates several endonucleases, including SLX1, MUS81-EME1, and XPF-ERCC1, to resolve DNA intermediates that arise during replication, recombination, and repair. Through its interaction network, Structure-specific endonuclease subunit SLX4 ensures proper processing of Holliday junctions, interstrand crosslinks, and stalled replication forks. The SLX4 antibody provides researchers with an essential reagent to study how this protein maintains genomic stability and prevents chromosomal abnormalities.

SLX4 serves as a platform that organizes structure-specific nucleases into a regulated complex. It promotes timely incision and resolution of DNA structures to prevent recombination defects and chromosome missegregation. The SLX4 antibody is particularly useful for investigating DNA damage responses following genotoxic stress, allowing visualization of nuclear foci formation at replication and repair sites. By detecting SLX4 expression and localization, researchers can monitor its participation in maintaining DNA replication fidelity and chromosomal stability.

Mutations in SLX4 cause Fanconi anemia complementation group P (FANCP), a rare hereditary disorder characterized by genomic instability, bone marrow failure, and cancer predisposition. These pathogenic variants disrupt SLX4's ability to interact with partner nucleases, compromising crosslink repair. The SLX4 antibody supports studies of Fanconi anemia mechanisms and helps identify defects in DNA repair pathways that arise from SLX4 dysfunction. Analysis of Structure-specific endonuclease subunit SLX4 expression also provides insight into its regulatory interactions with checkpoint proteins such as ATR and FANCD2.

In addition to its nuclear repair functions, SLX4 has roles in telomere maintenance, homologous recombination, and mitotic checkpoint regulation. The SLX4 antibody enables mapping of these diverse activities by revealing subcellular localization dynamics during the cell cycle. Western blot and immunofluorescence experiments with this antibody show how SLX4 expression fluctuates in S-phase and peaks during mitosis, correlating with its role in resolving replication intermediates.

Because genomic stability is a cornerstone of cancer prevention, SLX4 has gained increasing relevance in oncology. Altered expression levels may influence tumor sensitivity to DNA-damaging agents such as cisplatin. The SLX4 antibody supplied by NSJ Bioreagents supports research in cancer biology, DNA repair, and chromatin organization. With its validated specificity, the reagent allows consistent detection of Structure-specific endonuclease subunit SLX4 across assays, facilitating mechanistic studies on DNA repair regulation, genome maintenance, and disease pathogenesis.

UniProt: Q8IY92

Pathways: DNA Damage Repair