GLI2 antibody (AA 46-1398)

Catalog No. ABIN7875109

The Rabbit Polyclonal anti-GLI2 antibody (ABIN7875109) specifically detects GLI2 in WB, ELISA, IHC (p), IF and FACS. The antibody is reactive with Human and Rat samples.

Quick Overview for GLI2 antibody (AA 46-1398) (ABIN7875109)

Target: GLI2 (GLI Family Zinc Finger 2 (GLI2))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLI2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-GLI2 Antibody

Binding Specificity: AA 46-1398

Purpose: GLI2 Antibody / GLI family zinc finger protein 2

Purification: Antigen affinity purified

Immunogen: An E.coli-derived human recombinant protein (A46-A1398) was used as the immunogen for the GLI2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the GLI2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the GLI2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for GLI2

Target: GLI2 (GLI Family Zinc Finger 2 (GLI2))

Alternative Name: GLI2

Background: GLI2 (Gli-Kruppel Family Member 2), also called ONCOGENE GLI2, is a protein that in humans is encoded by the GLI2 gene. Sequencing of GLI cDNA clones showed the presence of 5 tandem zinc fingers connected by histidine-cysteine links, which indicated that the gene belongs to the family of zinc finger genes related to Kruppel (Kr). The Drosophila gene Kr is a member of the gap class of segmentation genes, thoracic and anterior abdominal segments fail to form in Kr mutant embryos. By fluorescence in situ hybridization, Matsumoto et al. (1996) refined the assignment of the GLI2 gene to chromosome 2q14. Roessler et al. (2005) showed that GLI2-delta-N exhibited potent transcriptional activity in vivo: overexpression in mouse skin led to the formation of hedgehog-independent epithelial downgrowths resembling basal cell carcinomas, which in humans are associated with constitutive hedgehog signaling.

UniProt: P10070

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis