RAG2 antibody (AA 46-503)

Catalog No. ABIN7875121

The Rabbit Polyclonal anti-RAG2 antibody (ABIN7875121) specifically detects RAG2 in WB, ELISA and FACS. The antibody is reactive with Human and Mouse samples.

Quick Overview for RAG2 antibody (AA 46-503) (ABIN7875121)

Target: RAG2 (Recombination Activating Gene 2 (RAG2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAG2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-RAG2 Antibody

Binding Specificity: AA 46-503

Purpose: RAG2 Antibody / V(D)J recombination-activating protein 2

Purification: Antigen affinity purified

Immunogen: E. coli-derived recombinant human protein (amino acids F46-K503) was used as the immunogen for the RAG2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the RAG2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the RAG2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for RAG2

Target: RAG2 (Recombination Activating Gene 2 (RAG2))

Alternative Name: RAG2

Background: Recombination activating gene 2, also known as RAG-2, is a protein that in humans is encoded by the RAG2 gene. This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms.

UniProt: P55895

Pathways: Chromatin Binding, Production of Molecular Mediator of Immune Response