CIT antibody (AA 470-1505)

Catalog No. ABIN7875167

This Rabbit Polyclonal antibody specifically detects CIT in ELISA, WB and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CIT antibody (AA 470-1505) (ABIN7875167)

Target: CIT (Citron (Rho-Interacting, serine/threonine Kinase 21) (CIT))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CIT antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-CIT Antibody

Binding Specificity: AA 470-1505

Purpose: CIT Antibody / Citron Rho-interacting kinase

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human CIT recombinant protein (Position: Q470-H1505) was used as the immunogen for the CIT antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the CIT antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the CIT antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for CIT

Target: CIT (Citron (Rho-Interacting, serine/threonine Kinase 21) (CIT))

Alternative Name: CIT

Background: CIT antibody detects Citron Rho-interacting kinase, a serine/threonine kinase that regulates cytokinesis and neuronal development. CIT functions downstream of Rho family GTPases to coordinate actomyosin contractility and midbody formation during cell division. The CIT antibody is used in cell cycle, cytoskeletal, and neurodevelopmental research to study contractile ring organization and cytokinetic abscission.

CIT is encoded by the CIT gene located on human chromosome 12q23.1. The protein is approximately 230 kilodaltons and contains an N-terminal kinase domain, a coiled-coil region, a Rho-binding domain, and a C-terminal citron homology region with multiple protein-protein interaction motifs. CIT localizes to the midbody during cytokinesis and to dendritic spines in postmitotic neurons.

The CIT antibody detects the 230 kilodalton protein in western blot analysis and reveals distinct midbody and dendritic staining patterns. CIT acts as a scaffold and kinase that phosphorylates substrates including myosin light chain and actin-regulatory proteins, coordinating contraction during cell division. Depletion of CIT disrupts cleavage furrow ingression and leads to multinucleation due to failed cytokinesis.

In the nervous system, Citron kinase contributes to dendrite formation, axon stability, and synaptic maturation. Mutations in CIT cause microcephaly and neuronal migration disorders due to abnormal mitotic spindle dynamics and impaired cell cycle progression in neural progenitors. CIT also interacts with RhoA and other GTPases, integrating cytoskeletal signaling with cortical actin organization.

CIT is a critical effector of Rho-mediated contractility and has been implicated in cancer cell proliferation and migration. NSJ Bioreagents provides a validated CIT antibody optimized for its applications, enabling detailed studies of cytokinesis, cytoskeletal remodeling, and neurodevelopmental regulation.

UniProt: O14578

Pathways: SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection