Sestrin 2 antibody (AA 48-341)

Catalog No. ABIN7875204

The Rabbit Polyclonal anti-Sestrin 2 antibody is suitable to detect Sestrin 2 in samples from Human. It has been validated for WB, ELISA, IHC, FACS and IF.

Quick Overview for Sestrin 2 antibody (AA 48-341) (ABIN7875204)

Target: Sestrin 2 (SESN2)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Sestrin 2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-Sestrin 2 Antibody

Binding Specificity: AA 48-341

Purpose: SESN2 Antibody / Sestrin 2

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human Sestrin 2/SESN2 recombinant protein (Position: I48-D341) was used as the immunogen for the SESN2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SESN2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SESN2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for Sestrin 2

Target: Sestrin 2 (SESN2)

Alternative Name: SESN2

Background: SESN2 antibody detects Sestrin 2, a stress-inducible metabolic regulator that protects cells from oxidative damage and maintains energy balance through AMPK and mTOR signaling. The UniProt recommended name is Sestrin 2 (SESN2). This cytoplasmic protein belongs to the sestrin family of stress-responsive factors that restore redox homeostasis and suppress excessive growth signaling under nutrient limitation or oxidative stress.

Functionally, SESN2 antibody identifies a 480-amino-acid protein induced by p53, FOXO, and ATF4 transcription factors in response to stress stimuli such as DNA damage, hypoxia, and reactive oxygen species. SESN2 activates AMP-activated protein kinase (AMPK) and inhibits mammalian target of rapamycin complex 1 (mTORC1) by interacting with the GATOR2 complex, thereby reducing anabolic activity and promoting autophagy. It also regenerates overoxidized peroxiredoxins, restoring cellular antioxidant capacity and maintaining metabolic equilibrium.

The SESN2 gene is located on chromosome 1p35.3 and is expressed in metabolically active tissues such as liver, skeletal muscle, and heart. Its expression levels increase during fasting, exercise, or oxidative stress, making SESN2 a crucial component of the cellular adaptive stress response.

Pathologically, dysregulation of SESN2 contributes to aging, metabolic disorders, and cancer. Reduced SESN2 activity promotes oxidative stress, mitochondrial dysfunction, and insulin resistance, while its overexpression has protective effects against neurodegeneration and tissue injury. Research using SESN2 antibody supports studies in autophagy, metabolism, and stress signaling pathways.

SESN2 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect oxidative stress regulators. NSJ Bioreagents provides SESN2 antibody reagents optimized for research in mTOR regulation, cellular homeostasis, and redox biology.

Structurally, Sestrin 2 contains a conserved catalytic domain with oxidoreductase-like motifs, though it lacks enzymatic activity. Instead, its structure supports binding to GATOR complexes and peroxiredoxins. This antibody facilitates detailed investigation of SESN2's role in energy sensing, antioxidant defense, and lifespan regulation.

UniProt: P58004