MADD antibody (AA 5-329)

Catalog No. ABIN7875304

The Rabbit Polyclonal anti-MADD antibody (ABIN7875304) specifically detects MADD in WB, ELISA and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for MADD antibody (AA 5-329) (ABIN7875304)

Target: MADD (MAP-Kinase Activating Death Domain (MADD))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MADD antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-MADD Antibody

Binding Specificity: AA 5-329

Purpose: MADD Antibody / MAP kinase-activating death domain

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human MADD recombinant protein (Position: K5-Y329) was used as the immunogen for the MADD antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the MADD antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the MADD antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for MADD

Target: MADD (MAP-Kinase Activating Death Domain (MADD))

Alternative Name: MADD

Background: MADD antibody detects MAP kinase-activating death domain protein, encoded by the MADD gene. MAP kinase-activating death domain protein is a multi-domain protein involved in apoptosis regulation, MAPK signaling, and vesicle trafficking. MADD antibody provides researchers with a specific reagent for studying TNF receptor signaling, neurodegeneration, and cancer.

MAP kinase-activating death domain protein interacts with TNF receptor 1 and other death receptors through its death domain, modulating apoptosis and survival. Research using MADD antibody has shown that it can function as an anti-apoptotic factor by inhibiting TNF-induced cell death, while simultaneously promoting MAP kinase signaling pathways that enhance proliferation. This dual role highlights MADD as a regulator of cell fate decisions.

Studies with MADD antibody have revealed that MADD is widely expressed but is particularly abundant in brain, endocrine tissues, and tumors. It is expressed as multiple isoforms through alternative splicing, including long and short forms that differ in their ability to regulate apoptosis and signaling. These isoforms allow MADD to act as a context-dependent modulator of signaling cascades.

Dysregulation of MAP kinase-activating death domain protein has been associated with cancer and neurodegenerative disease. Research using MADD antibody has shown that overexpression supports tumor growth by suppressing apoptosis, while mutations or altered expression in neurons contribute to neurodegenerative pathology. Elevated expression has also been observed in thyroid cancers and other tumors, positioning MADD as a potential biomarker and therapeutic target.

Beyond apoptosis, MADD participates in intracellular trafficking. Research using MADD antibody has demonstrated interactions with Rab3 and related GTPases, regulating synaptic vesicle release and exocytosis. This adds a neurobiological dimension to MADD function and explains its abundance in neurons.

MADD antibody is widely applied in western blotting, immunohistochemistry, and immunoprecipitation. Western blotting quantifies isoform expression across tissues, immunohistochemistry demonstrates tumor expression, and immunoprecipitation identifies receptor and GTPase interactions. These applications make MADD antibody indispensable for apoptosis, signaling, and cancer research.

By providing validated MADD antibody reagents, NSJ Bioreagents supports studies into TNF receptor pathways, apoptosis, and disease. Detection of MAP kinase-activating death domain protein provides researchers with insight into how death domain proteins regulate survival and pathology.

UniProt: Q8WXG6

Pathways: Caspase Cascade in Apoptosis