IRF3 antibody (AA 5-422)

Catalog No. ABIN7875310

The Rabbit Polyclonal anti-IRF3 antibody (ABIN7875310) specifically detects IRF3 in WB, ELISA, FACS and IHC (p). The antibody is reactive with Human samples.

Quick Overview for IRF3 antibody (AA 5-422) (ABIN7875310)

Target: IRF3 (Interferon Regulatory Factor 3 (IRF3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRF3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-IRF3 Antibody

Binding Specificity: AA 5-422

Purpose: IRF3 Antibody

Purification: Affinity purified

Immunogen: Recombinant human protein (amino acids K5-Q422) was used as the immunogen for the IRF3 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the IRF3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the IRF3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for IRF3

Target: IRF3 (Interferon Regulatory Factor 3 (IRF3))

Alternative Name: IRF3

Background: IRF3 (interferon regulatory factor 3) is a member of the interferon regulatory transcription factor (IRF) family. The IRF3 gene is mapped on 19q13.33. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. IRF3 plays an important role in the innate immune system's response to viral infection. Aggregated MAVS have been found to activate IRF3 dimerization. Although IRF3 increased transcriptional activity from an ISRE-containing promoter, expression of IRF3 as a Gal4 fusion protein did not activate expression of a chloramphenicol acetyltransferase (CAT) reporter gene containing repeats of the Gal4-binding sites. Translocation of IRF3 was accompanied by an increase in serine and threonine phosphorylation. The transcriptional activators CREBBP and EP300 coimmunoprecipitated with IRF3 only subsequent to viral infection, and the authors stated that these are also subunits of DRAF1.

UniProt: Q14653

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades