LRRC75A/FAM211A antibody (AA 52-292)
The Rabbit Polyclonal anti-LRRC75A/FAM211A antibody is suitable to detect LRRC75A/FAM211A in samples from Human. It has been validated for WB, ELISA and FACS.
Catalog No. ABIN7875558
$625.62
Plus shipping costs $50.00
100 μg
Shipping to:
United States
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Quick Overview for LRRC75A/FAM211A antibody (AA 52-292) (ABIN7875558)
Target
LRRC75A/FAM211A (LRRC75A)
(Leucine Rich Repeat Containing 75A (LRRC75A))
Reactivity
Human
Host
Rabbit
Clonality
Polyclonal
Conjugate
This LRRC75A/FAM211A antibody is un-conjugated
Application
Western Blotting (WB), ELISA, Flow Cytometry (FACS)
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Binding Specificity
- AA 52-292
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Purpose
- LRRC75A Antibody / FAM211A
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Purification
- Antigen affinity purified
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Immunogen
- An E.coli-derived human recombinant protein (amino acids H52-Q292) was used as the immunogen for the LRRC75A antibody.
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Isotype
- IgG
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Application Notes
- Optimal dilution of the LRRC75A antibody should be determined by the researcher.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
- 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
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Storage
- 4 °C,-20 °C
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Storage Comment
- After reconstitution, the LRRC75A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- LRRC75A/FAM211A (LRRC75A) (Leucine Rich Repeat Containing 75A (LRRC75A))
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Alternative Name
- LRRC75A
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Background
- Long noncoding RNAs (lncRNAs) play multiple key roles during inflammatory processes. A novel lncRNA identified by the high-throughput sequencing analysis was found significantly down-regulated in Escherichia coli-introduced cell model of bovine mastitis. Given that this lncRNA consists of the antisense of leucine-rich repeat-containing protein 75A (LRRC75A), it was named LRRC75A antisense lncRNA1 (LRRC75A-AS1). The expression of LRRC75A-AS1 was down-regulated in bovine mammary epithelial cells and mammary tissues under inflammatory condition. Knockout (KO) of LRRC75A-AS1 by CRISPR-Cas9 system in bovine mammary alveolar cell-T (MAC-T) cell line could enhance expressions of tight junction (TJ) proteins Claudin-1, Occludin and ZO-1, reduce cell monolayer permeability, and inhibit Staphylococcus aureus adhesion and invasion. Meanwhile, it also down-regulated expressions of inflammatory factors and attenuated activation of NF-?B pathway. Similarly, knockdown of LRRC75A caused the changes as LRRC75A-AS1 KO did, while overexpression of LRRC75A enabled the opposite effects. TJ of epithelioid cells barriers the pathogenic microorganisms outside during inflammation, in which LRRC75A-AS1 can regulate the expression of TJ proteins through LRRC75A, affecting the development of inflammation.
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UniProt
- Q8NAA5
Target
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