LRRC75A/FAM211A antibody (AA 52-292) (Biotin)

Catalog No. ABIN8005556

This Rabbit Polyclonal antibody specifically detects LRRC75A/FAM211A in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for LRRC75A/FAM211A antibody (AA 52-292) (Biotin) (ABIN8005556)

Target: LRRC75A/FAM211A (LRRC75A) (Leucine Rich Repeat Containing 75A (LRRC75A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LRRC75A/FAM211A antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-LRRC75A/FAM211A Antibody (Biotin)

Binding Specificity: AA 52-292

Purpose: Anti-LRRC75A Antibody Biotin Conjugated

Cross-Reactivity (Details): No cross reactivity with other proteins.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human LRRC75A recombinant protein (Position: H52-Q292).

Isotype: IgG

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for LRRC75A/FAM211A

Target: LRRC75A/FAM211A (LRRC75A) (Leucine Rich Repeat Containing 75A (LRRC75A))

Alternative Name: LRRC75A

Background:

Background: Long noncoding RNAs (lncRNAs) play multiple key roles during inflammatory processes. A novel lncRNA identified by the high-throughput sequencing analysis was found significantly down-regulated in Escherichia coli-introduced cell model of bovine mastitis. Given that this lncRNA consists of the antisense of leucine-rich repeat-containing protein 75A (LRRC75A), it was named LRRC75A antisense lncRNA1 (LRRC75A-AS1). The expression of LRRC75A-AS1 was down-regulated in bovine mammary epithelial cells and mammary tissues under inflammatory condition. Knockout (KO) of LRRC75A-AS1 by CRISPR-Cas9 system in bovine mammary alveolar cell-T (MAC-T) cell line could enhance expressions of tight junction (TJ) proteins Claudin-1, Occludin and ZO-1, reduce cell monolayer permeability, and inhibit Staphylococcus aureus adhesion and invasion. Meanwhile, it also down-regulated expressions of inflammatory factors and attenuated activation of NF-κB pathway. Similarly, knockdown of LRRC75A caused the changes as LRRC75A-AS1 KO did, while overexpression of LRRC75A enabled the opposite effects. TJ of epithelioid cells barriers the pathogenic microorganisms outside during inflammation, in which LRRC75A-AS1 can regulate the expression of TJ proteins through LRRC75A, affecting the development of inflammation.

Gene Full Name: leucine rich repeat containing 75A

Gene ID: 388341

UniProt: Q8NAA5