TNS3 antibody (AA 592-869)

Catalog No. ABIN7875930

The Rabbit Polyclonal anti-TNS3 antibody is suitable to detect TNS3 in samples from Human. It has been validated for IHC, WB, ELISA, IF, IP, ICC and FACS.

Quick Overview for TNS3 antibody (AA 592-869) (ABIN7875930)

Target: TNS3 (Tensin 3 (TNS3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNS3 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-TNS3 Antibody

Binding Specificity: AA 592-869

Purpose: TNS3 Antibody / Tensin 3

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human TNS3 recombinant protein (Position: Q592-E869) was used as the immunogen for the TNS3 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the TNS3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the TNS3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for TNS3

Target: TNS3 (Tensin 3 (TNS3))

Alternative Name: TNS3

Background: TNS3 antibody detects Tensin-3, a cytoskeletal adaptor protein that links integrins to the actin cytoskeleton and participates in cell adhesion, migration, and signal transduction. Encoded by the TNS3 gene on chromosome 7p12.3, this protein is part of the tensin family, which plays key roles in focal adhesion dynamics and mechanical signal integration. Tensin-3 contributes to the structural connection between integrin-mediated extracellular matrix adhesion sites and intracellular actin filaments, regulating cellular shape and motility.

Structurally, Tensin-3 contains Src homology 2 (SH2) and phosphotyrosine-binding (PTB) domains that interact with phosphorylated tyrosine residues on focal adhesion proteins and with integrin cytoplasmic tails. Through these domains, TNS3 facilitates recruitment of signaling molecules, including Src family kinases, PI3K, and FAK, at adhesion sites. It also modulates Rho GTPase signaling, influencing actin organization and cell polarity.

The TNS3 antibody is widely used in cancer biology, cytoskeletal research, and cell signaling studies to investigate adhesion remodeling and integrin signaling. Western blot analysis detects a 150-160 kilodalton band corresponding to Tensin-3, while immunofluorescence reveals focal adhesion and cytoplasmic localization. This antibody supports studies examining adhesion turnover, migration, and mechanotransduction pathways in normal and malignant cells.

Tensin-3 expression is frequently altered in cancer, where it influences metastatic potential and invasion by regulating focal adhesion stability and actin remodeling. Overexpression promotes invasive behavior in several carcinoma types, while loss of function impairs migration and cell spreading. The TNS3 antibody provides a reliable reagent for exploring adhesion-mediated signaling and tumor cell motility. NSJ Bioreagents offers this antibody validated for western blotting, immunohistochemistry, and immunofluorescence, ensuring reproducible detection for cytoskeletal and signaling research.

UniProt: Q68CZ2