SAP130 antibody (AA 728-1181)

Catalog No. ABIN7876645

The Rabbit Polyclonal anti-SAP130 antibody (ABIN7876645) specifically detects SAP130 in WB, ELISA, IHC (p), IF and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for SAP130 antibody (AA 728-1181) (ABIN7876645)

Target: SAP130 (Sin3A-Associated Protein, 130kDa (SAP130))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SAP130 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-SAP130 Antibody

Binding Specificity: AA 728-1181

Purpose: SAP130 Antibody / SF3B3

Purification: Antigen affinity purified

Immunogen: E. coli-derived recombinant human protein (amino acids R728-Q1181) was used as the immunogen for the SAP130 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SAP130 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SAP130 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for SAP130

Target: SAP130 (Sin3A-Associated Protein, 130kDa (SAP130))

Alternative Name: SAP130

Background: Splicing factor 3B subunit 3 is a protein that in humans is encoded by the SF3B3 gene. This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair.

UniProt: Q15393