RAB1A antibody (AA 74-199)

Catalog No. ABIN7876703

This Rabbit Polyclonal antibody specifically detects RAB1A in WB, ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for RAB1A antibody (AA 74-199) (ABIN7876703)

Target: RAB1A (RAB1A, Member RAS Oncogene Family (RAB1A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAB1A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-RAB1A Antibody

Binding Specificity: AA 74-199

Purpose: RAB1A Antibody / Ras-related protein Rab-1A

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human RAB1A recombinant protein (Position: R74-Q199) was used as the immunogen for the RAB1A antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the RAB1A antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the RAB1A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for RAB1A

Target: RAB1A (RAB1A, Member RAS Oncogene Family (RAB1A))

Alternative Name: RAB1A

Background: RAB1A antibody detects Ras-related protein Rab-1A, a small GTPase involved in vesicular trafficking between the endoplasmic reticulum (ER) and Golgi apparatus. The UniProt recommended name is Ras-related protein Rab-1A (RAB1A). This essential member of the Rab family regulates the formation, movement, and docking of transport vesicles that ensure protein secretion and Golgi organization.

Functionally, RAB1A antibody identifies a 205-amino-acid GTP-binding protein that cycles between active GTP-bound and inactive GDP-bound states. Active RAB1A recruits effector proteins such as p115, GM130, and COPII components to mediate vesicle tethering and fusion at the cis-Golgi membrane. It coordinates early secretory pathway events and maintains ER-to-Golgi transport fidelity, supporting proper protein maturation and trafficking.

The RAB1A gene is located on chromosome 2p14 and is ubiquitously expressed in eukaryotic cells, with particularly high expression in secretory tissues. Its activity is regulated by GEFs (guanine nucleotide exchange factors) and GAPs (GTPase-activating proteins) that control the GTP/GDP cycle and subcellular localization.

Pathologically, RAB1A dysregulation affects autophagy, neurodegeneration, and cancer progression. Overexpression enhances cell proliferation and Golgi reorganization in tumor cells, while impaired RAB1A function contributes to ER stress and defective autophagosome formation. Research using RAB1A antibody supports studies in membrane trafficking, Golgi function, and oncogenic signaling.

RAB1A antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect small GTPases. NSJ Bioreagents provides RAB1A antibody reagents optimized for research in vesicle transport, protein secretion, and cellular trafficking dynamics.

Structurally, Ras-related protein Rab-1A contains conserved GTP-binding motifs (GxxxxGK[S/T], DxxG, NKxD, and SAx) and a C-terminal geranylgeranylation site that anchors it to membranes. Its switch regions undergo conformational changes upon GTP binding, enabling interaction with effectors that mediate vesicle transport. This antibody allows exploration of RAB1A's role in ER-Golgi trafficking and its contribution to secretory pathway regulation.

UniProt: P62820

Pathways: SARS-CoV-2 Protein Interactome