MED12 antibody (AA 752-1974)

Catalog No. ABIN7876734

The Rabbit Polyclonal anti-MED12 antibody is suitable to detect MED12 in samples from Human. It has been validated for ELISA and WB.

Quick Overview for MED12 antibody (AA 752-1974) (ABIN7876734)

Target: MED12 (Mediator Complex Subunit 12 (MED12))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED12 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details anti-MED12 Antibody

Binding Specificity: AA 752-1974

Purpose: MED12 Antibody / Mediator complex subunit 12

Purification: Antigen affinity purified

Immunogen: An E.coli-derived human recombinant protein (Q752-Q1974) was used as the immunogen for the MED12 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the MED12 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the MED12 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for MED12

Target: MED12 (Mediator Complex Subunit 12 (MED12))

Alternative Name: MED12

Background: Mediator of RNA polymerase II transcription, subunit 12 homolog (S. cerevisiae), also known as MED12, is a human gene found on the X chromosome. The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex which contains the protein encoded by this gene, mediator complex subunit 12 (MED12), along with MED13, CDK8 kinase, and cyclin C. The CDK8 subcomplex modulates Mediator-polymerase II interactions and thereby regulates transcription initiation and reinitation rates. The MED12 protein is essential for activating CDK8 kinase. Defects in this gene cause X-linked Opitz-Kaveggia syndrome, also known as FG syndrome, and Lujan-Fryns syndrome.

UniProt: Q93074

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Stem Cell Maintenance, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha, Tube Formation