NGEF antibody (AA 82-627)

Catalog No. ABIN7876924

The Rabbit Polyclonal anti-NGEF antibody (ABIN7876924) specifically detects NGEF in WB, ELISA and FACS. The antibody is reactive with Human samples.

Quick Overview for NGEF antibody (AA 82-627) (ABIN7876924)

Target: NGEF (Neuronal Guanine Nucleotide Exchange Factor (NGEF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NGEF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-NGEF Antibody

Binding Specificity: AA 82-627

Purpose: NGEF Antibody / Ephexin 1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human NGEF recombinant protein (Position: E82-Q627) was used as the immunogen for the NGEF antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the NGEF antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the NGEF antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for NGEF

Target: NGEF (Neuronal Guanine Nucleotide Exchange Factor (NGEF))

Alternative Name: NGEF

Background: NGEF antibody detects Ephexin 1, encoded by the NGEF gene. Ephexin 1 is a guanine nucleotide exchange factor that regulates Rho family GTPases, controlling cytoskeletal dynamics, neuronal signaling, and axon guidance. NGEF antibody provides researchers with a specialized reagent for studying neuronal development, synaptic plasticity, and cell signaling.

Ephexin 1 is activated by ephrin receptor signaling, linking extracellular cues to intracellular Rho GTPase activity. Research using NGEF antibody has shown that it catalyzes GDP to GTP exchange on RhoA, Rac1, and Cdc42, thereby modulating actin cytoskeleton remodeling. These activities govern axon guidance during development, influencing neuronal wiring and brain connectivity.

Studies with NGEF antibody have revealed that Ephexin 1 is enriched in neurons, particularly in growth cones and synaptic regions. Its regulation by ephrin receptors ensures that axons respond appropriately to repulsive or attractive cues. This makes NGEF a critical player in neural circuit formation and brain development.

Dysregulation of Ephexin 1 has been linked to neurological disorders, including developmental delay, intellectual disability, and autism spectrum disorders. Research using NGEF antibody has shown that mutations or altered expression disrupt axon guidance and synaptic function, impairing neuronal communication. These findings highlight its clinical significance.

Beyond the nervous system, NGEF may play roles in cytoskeletal regulation in non-neuronal cells. Research using NGEF antibody has suggested functions in cell migration, immune cell trafficking, and tumor invasion. These roles broaden its biological relevance beyond neurodevelopment.

NGEF antibody is widely applied in immunohistochemistry, western blotting, and immunofluorescence. Immunohistochemistry highlights expression in brain tissue, western blotting quantifies protein levels across developmental stages, and immunofluorescence reveals subcellular localization at synapses. These applications make NGEF antibody indispensable for neurobiology research.

By supplying validated NGEF antibody reagents, NSJ Bioreagents supports studies into axon guidance, synaptic signaling, and disease. Detection of Ephexin 1 provides researchers with insight into how guanine nucleotide exchange factors regulate neuronal wiring and function.

UniProt: Q8N5V2

Pathways: Neurotrophin Signaling Pathway