NSUN7 antibody (AA 83-457)

Catalog No. ABIN7876943

This Rabbit Polyclonal antibody specifically detects NSUN7 in ELISA, WB and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for NSUN7 antibody (AA 83-457) (ABIN7876943)

Target: NSUN7 (NOP2/Sun Domain Family, Member 7 (NSUN7))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NSUN7 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-NSUN7 Antibody

Binding Specificity: AA 83-457

Purpose: NSUN7 Antibody / NOP2/Sun RNA methyltransferase family member 7

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human NSUN7 recombinant protein (Position: D83-Q457) was used as the immunogen for the NSUN7 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the NSUN7 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the NSUN7 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for NSUN7

Target: NSUN7 (NOP2/Sun Domain Family, Member 7 (NSUN7))

Alternative Name: NSUN7

Background: NSUN7 antibody detects NOP2/Sun RNA methyltransferase family member 7, an enzyme responsible for methylating cytosine residues in RNA molecules, particularly within mitochondrial and spermatozoal transcripts. Encoded by the NSUN7 gene on chromosome 4q26, this enzyme belongs to the RNA cytosine methyltransferase family, which regulates RNA stability, translation, and processing through site-specific methylation. NSUN7 plays key roles in sperm motility, mitochondrial metabolism, and epigenetic control of gene expression.

Structurally, NSUN7 is a 721-amino-acid mitochondrial and cytoplasmic protein of approximately 80 kilodaltons containing a conserved catalytic cysteine motif and an S-adenosylmethionine (SAM)-binding domain required for methyl group transfer. It is expressed predominantly in testes, brain, and metabolic tissues, reflecting its involvement in mitochondrial gene regulation and male fertility. The enzyme localizes to the mitochondrial matrix and cytoplasmic ribonucleoprotein complexes, where it modifies tRNAs and mRNAs.

The NSUN7 antibody is widely used in epigenetics, reproductive biology, and mitochondrial research to study RNA methylation, translational regulation, and sperm function. Western blot analysis detects an 80 kilodalton band corresponding to NSUN7, while immunofluorescence reveals cytoplasmic and mitochondrial staining in germ cells and metabolic tissues. This antibody supports studies examining RNA modification-dependent regulation of gene expression.

Functionally, NSUN7 contributes to mitochondrial energy homeostasis and sperm motility by methylating specific RNA molecules required for mitochondrial translation. Mutations in NSUN7 cause male infertility due to defective sperm motility and impaired mitochondrial activity. Emerging evidence suggests that RNA methylation by NSUN7 influences cellular energy balance and oxidative stress responses beyond the reproductive system. The NSUN7 antibody provides a powerful reagent for studying RNA modification pathways, mitochondrial RNA biology, and epigenetic regulation of metabolism. NSJ Bioreagents validates this antibody for its applications, ensuring reproducible detection across RNA methylation studies.

UniProt: Q8NE18