PYHIN1 antibody (AA 86-488)

Catalog No. ABIN7877000

This Rabbit Polyclonal antibody specifically detects PYHIN1 in WB, ELISA, FACS, IHC, ICC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PYHIN1 antibody (AA 86-488) (ABIN7877000)

Target: PYHIN1 (Pyrin and HIN Domain Family, Member 1 (PYHIN1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PYHIN1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-PYHIN1 Antibody

Binding Specificity: AA 86-488

Purpose: PYHIN1 Antibody / Pyrin and HIN domain-containing protein 1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human PYHIN1 recombinant protein (Position: K86-H488) was used as the immunogen for the PYHIN1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PYHIN1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the PYHIN1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PYHIN1

Target: PYHIN1 (Pyrin and HIN Domain Family, Member 1 (PYHIN1))

Alternative Name: PYHIN1

Background: PYHIN1 antibody detects Pyrin and HIN domain-containing protein 1, a nuclear and cytoplasmic signaling protein involved in immune regulation, cell cycle control, and apoptosis. Encoded by the PYHIN1 gene on chromosome 1q23.1, this protein belongs to the HIN-200 (hematopoietic interferon-inducible nuclear) family of interferon-inducible proteins, which are characterized by an N-terminal pyrin domain and a C-terminal HIN-200 DNA-binding domain. These domains mediate protein-protein and protein-DNA interactions that regulate immune transcription and DNA damage response pathways.

PYHIN1 functions primarily as a transcriptional regulator and sensor of foreign or damaged DNA. It can bind double-stranded DNA through its HIN domain and modulate transcription of interferon-stimulated genes. In the nucleus, PYHIN1 interacts with transcriptional co-regulators to influence gene expression linked to antiviral defense and tumor suppression. In the cytoplasm, it contributes to inflammasome regulation and apoptotic signaling. Expression of PYHIN1 is strongly induced by type I interferons and inflammatory cytokines, linking it to host defense and immune surveillance.

The PYHIN1 antibody is used in immunology, oncology, and molecular biology to investigate DNA sensing, innate immune activation, and cell cycle regulation. Western blot analysis identifies a 74 kilodalton band corresponding to PYHIN1, while immunofluorescence shows both nuclear and cytoplasmic staining depending on cell type and activation state. Because PYHIN1 acts at the interface of DNA sensing and transcriptional control, the antibody is a valuable tool for studying interferon signaling and genomic stability mechanisms.

Beyond innate immunity, PYHIN1 plays roles in tumor suppression and apoptosis. Its expression inhibits cell proliferation by interfering with E2F transcriptional activity and promoting p53-dependent growth arrest. Loss of PYHIN1 has been reported in several cancers, suggesting that it functions as a tumor suppressor gene. The PYHIN1 antibody enables researchers to explore these functions and track its expression across different physiological and disease contexts. NSJ Bioreagents provides this antibody validated for its applications, ensuring specific and reproducible detection across model systems.

UniProt: Q6K0P9