ASH2L antibody (AA 95-584)

Catalog No. ABIN7877225

This Rabbit Polyclonal antibody specifically detects ASH2L in IF and IHC (p). It exhibits reactivity toward Zebrafish (Danio rerio).

Quick Overview for ASH2L antibody (AA 95-584) (ABIN7877225)

Target: ASH2L (Set1/Ash2 Histone Methyltransferase Complex Subunit ASH2 (ASH2L))

Reactivity: Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ASH2L antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ASH2L Antibody

Binding Specificity: AA 95-584

Purpose: Zebrafish Ash2l Antibody / ASH2-like protein

Purification: Antigen affinity chromatography

Immunogen: An E.coli-derived zebrafish Ash2l recombinant protein (amino acids N95-G584) was used as the immunogen for the Zebrafish Ash2l antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Optimal dilution of the Zebrafish Ash2l antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Zebrafish Ash2l antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for ASH2L

Target: ASH2L (Set1/Ash2 Histone Methyltransferase Complex Subunit ASH2 (ASH2L))

Alternative Name: Ash2l

Background: Set1/Ash2 histone methyltransferase complex subunit ASH2 is an enzyme that in humans is encoded by the ASH2L gene. The Set1 histone methyltransferase protein was first identified in yeast as part of the Set1/COMPASS histone methyltransferase complex, which methylates histone H3 at Lys4 and functions as a transcriptional co-activator. While yeast contain only one known Set1 protein, six Set1-related proteins exist in mammals: SET1A, SET1B, MLL1, MLL2, MLL3, and MLL4, all of which assemble into COMPASS-like complexes and methylate histone H3 at Lys4. These Set1-related proteins are each found in distinct protein complexes, all of which share the common subunits WDR5, RBBP5, ASH2L, CXXC1 and DPY30. These subunits are required for proper complex assembly and modulation of histone methyltransferase activity. MLL1 and MLL2 complexes contain the additional protein subunit, menin. Like yeast Set1, all six Set1-related mammalian proteins methylate histone H3 at Lys4. MLL translocations are found in a large number of hematological malignancies, suggesting that Set1/COMPASS histone methyltransferase complexes play a critical role in leukemogenesis.