WEE1 antibody (AA 96-130)

Catalog No. ABIN7877241

The Rabbit Polyclonal anti-WEE1 antibody is suitable to detect WEE1 in samples from Human. It has been validated for WB.

Quick Overview for WEE1 antibody (AA 96-130) (ABIN7877241)

Target: WEE1 (WEE1 Homolog (S. Pombe) (WEE1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WEE1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-WEE1 Antibody

Binding Specificity: AA 96-130

Purpose: Wee1 Antibody

Purification: Antigen affinity

Immunogen: A portion of amino acids 96-130 from human Wee1 was used as the immunogen for the Wee1 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the Wee1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Wee1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for WEE1

Target: WEE1 (WEE1 Homolog (S. Pombe) (WEE1))

Alternative Name: Wee1

Background: Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation. [UniProt]

UniProt: P30291

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase