Cathepsin S antibody (C-Term)

Catalog No. ABIN7877329

The Rabbit Polyclonal anti-Cathepsin S antibody (ABIN7877329) specifically detects Cathepsin S in WB and FACS. The antibody is reactive with Human samples.

Quick Overview for Cathepsin S antibody (C-Term) (ABIN7877329)

Target: Cathepsin S (CTSS)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cathepsin S antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-Cathepsin S Antibody

Binding Specificity: C-Term

Purpose: CTSS Antibody / Cathepsin S

Purification: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human CTSS was used as the immunogen for the CTSS antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the CTSS antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the CTSS antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for Cathepsin S

Target: Cathepsin S (CTSS)

Alternative Name: CTSS

Background: CTSS antibody detects Cathepsin S, a lysosomal cysteine protease that degrades extracellular matrix and participates in antigen processing. The UniProt recommended name is Cathepsin S (CTSS). This proteolytic enzyme plays a central role in endosomal protein degradation and MHC class II-mediated antigen presentation, particularly within antigen-presenting cells such as macrophages and dendritic cells.

Functionally, CTSS antibody identifies a 331-amino-acid protein synthesized as an inactive proenzyme that undergoes autocatalytic cleavage to form an active mature enzyme in acidic endosomes. Cathepsin S hydrolyzes invariant chain peptides to allow loading of antigenic peptides onto MHC class II molecules, supporting adaptive immune responses. It also contributes to extracellular matrix remodeling during inflammation and immune cell migration.

The CTSS gene is located on chromosome 1q21.3 and is highly expressed in lymphoid tissues and mononuclear phagocytes. Cathepsin S activity is regulated by cystatin protease inhibitors and by pH -dependent activation within the lysosomal environment. Its stability at neutral pH distinguishes it from other cathepsins, enabling extracellular activity during inflammation.

Pathologically, excessive CTSS activity has been associated with autoimmune diseases, atherosclerosis, and cancer. Inhibiting CTSS reduces antigen presentation and inflammatory damage, making it a therapeutic target for chronic inflammatory disorders. Research using CTSS antibody supports studies in immunology, inflammation, and protease biology.

CTSS antibody is validated for western blotting, immunohistochemistry, and enzymatic assays to detect Cathepsin S in immune tissues and cultured cells. NSJ Bioreagents provides high-quality CTSS antibody reagents optimized for proteolytic enzyme and immune pathway research.

Structurally, Cathepsin S belongs to the papain family of cysteine proteases and contains a catalytic dyad of cysteine and histidine within its active site. This antibody facilitates detection and quantification of CTSS in studies of antigen processing, protease regulation, and immune signaling.

UniProt: P25774

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades