GGN antibody (C-Term)

Catalog No. ABIN7877383

The Rabbit Polyclonal anti-GGN antibody (ABIN7877383) specifically detects GGN in WB and FACS. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for GGN antibody (C-Term) (ABIN7877383)

Target: GGN (Gametogenetin (GGN))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GGN antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-GGN Antibody

Binding Specificity: C-Term

Purpose: GGN Antibody / Gametogenetin

Purification: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human GGN was used as the immunogen for the CGN antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the GGN antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the GGN antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for GGN

Target: GGN (Gametogenetin (GGN))

Alternative Name: GGN

Background: GGN antibody detects Gametogenetin (also known as Gametogenetin protein), a testis-enriched protein that participates in germ cell differentiation and DNA repair during spermatogenesis. Encoded by the GGN gene on chromosome 19q13.33, this protein is predominantly expressed in spermatocytes and spermatids, where it contributes to chromatin organization, meiotic progression, and sperm maturation. Gametogenetin contains multiple coiled-coil domains and nuclear localization sequences, enabling interactions with DNA repair proteins and chromatin-associated complexes. It forms part of a multi-protein network essential for maintaining genome stability during meiosis.

Gametogenetin is highly conserved across mammals and plays a crucial role in spermatogenic cell viability. Studies have shown that GGN interacts with Fanconi anemia proteins and DNA polymerases, suggesting a role in homologous recombination repair. Disruption of GGN function results in defective sperm development and infertility in animal models. Its expression pattern, confined largely to the testis, makes it a useful molecular marker for germ cell differentiation and testicular function. The GGN antibody provides a key reagent for studying reproductive biology, meiotic regulation, and DNA damage response mechanisms in germ cells.

In addition to reproductive functions, low levels of GGN expression have been detected in certain somatic tissues, including brain and kidney, though its physiological significance there remains uncertain. The protein is localized primarily in the nucleus but may also associate with cytoplasmic vesicles during spermiogenesis. Western blot analysis using the GGN antibody identifies a ~70 kilodalton band, while immunohistochemistry reveals nuclear staining in spermatogenic cells within seminiferous tubules. The antibody is useful for distinguishing stages of spermatogenesis and assessing germ cell health in both normal and pathological conditions.

Because GGN interacts with DNA repair proteins, its dysregulation may contribute to genomic instability or subfertility. The GGN antibody enables detailed investigation of gametogenic processes and molecular pathways that maintain genome integrity in reproductive cells. NSJ Bioreagents provides this antibody validated for its applications, ensuring reliable performance in developmental and reproductive biology research.

UniProt: Q86UU5