KRT14 antibody (C-Term)

Catalog No. ABIN7877408

This Mouse Monoclonal antibody specifically detects KRT14 in IHC (p). It exhibits reactivity toward Human.

Quick Overview for KRT14 antibody (C-Term) (ABIN7877408)

Target: KRT14 (Keratin 14 (KRT14))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT14 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM263

Product Details anti-KRT14 Antibody

Binding Specificity: C-Term

Purpose: Anti-Cytokeratin 14 Antibody

Purification: Protein G affinity chromatography

Immunogen: A 15 amino acid peptide from the C-terminus of human keratin 14 was used as the immunogen for this anti-Cytokeratin 14 antibody.

Isotype: IgG3, kappa

Application Details

Application Notes: The optimal dilution of the anti-Cytokeratin 14 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the anti-Cytokeratin 14 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for KRT14

Target: KRT14 (Keratin 14 (KRT14))

Alternative Name: Cytokeratin 14

Background: Cytokeratin 14 (CK14) belongs to the type I (or A or acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5 (type II or B or basic). CK14 is found in basal cells of squamous epithelia, some glandular epithelia, myoepithelium, and mesothelial cells. Anti-CK14 is useful in differentiating squamous cell carcinomas from poorly differentiated epithelial tumors. Anti-CK14 is one of the specific basal markers for distinguishing between basal and non-basal subtypes of breast carcinomas. Anti-CK14 is also a good marker for differentiation of intraductal from invasive salivary duct carcinoma by the positive staining of basal cells surrounding the in-situ neoplasm as well as for differentiation of benign prostate from prostate carcinoma. Furthermore, this antibody has been useful in separating oncocytic tumors of the kidney from its renal mimics, and in identifying metaplastic carcinomas of the breast.

UniProt: P02533