MYL3/CMLC1 antibody (C-Term)

Catalog No. ABIN7877434

The Rabbit Polyclonal anti-MYL3/CMLC1 antibody (ABIN7877434) specifically detects MYL3/CMLC1 in WB. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for MYL3/CMLC1 antibody (C-Term) (ABIN7877434)

Target: MYL3/CMLC1 (MYL3) (Myosin, Light Chain 3 (MYL3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MYL3/CMLC1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MYL3/CMLC1 Antibody

Binding Specificity: C-Term

Purpose: MYL3 Antibody / Myosin light chain 3

Purification: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human MYL3 was used as the immunogen for the MYL3 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the MYL3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the MYL3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for MYL3/CMLC1

Target: MYL3/CMLC1 (MYL3) (Myosin, Light Chain 3 (MYL3))

Alternative Name: MYL3

Background: MYL3 antibody detects Myosin light chain 3, a contractile protein component of cardiac and skeletal muscle that modulates actin-myosin interactions during muscle contraction. The UniProt recommended name is Myosin light chain 3 (MYL3). This protein binds to myosin heavy chains and contributes to force generation and sarcomere stability in striated muscle fibers.

Functionally, MYL3 antibody identifies a 151-amino-acid calcium-binding protein localized to the myofilament's thick filament region. MYL3 interacts with the myosin heavy chain alpha isoform and regulates actomyosin ATPase activity through conformational changes induced by calcium binding. It plays a key role in sarcomere assembly, contractile force regulation, and muscle fiber maintenance.

The MYL3 gene is located on chromosome 3p21.31 and is expressed predominantly in ventricular cardiac tissue and slow-twitch skeletal muscles. MYL3 is essential for normal heart contractility and mechanical stability under physiological stress.

Pathologically, mutations in MYL3 cause familial hypertrophic cardiomyopathy and restrictive cardiomyopathy by altering calcium binding and myosin-actin interactions. Defects in MYL3 impair contractile performance and promote myofibrillar disarray. Research using MYL3 antibody supports studies in cardiac physiology, muscle biochemistry, and hereditary myopathies.

MYL3 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect sarcomeric proteins. NSJ Bioreagents provides MYL3 antibody reagents optimized for research in cardiomyocyte biology, muscle contraction, and structural myopathy studies.

Structurally, Myosin light chain 3 belongs to the EF-hand superfamily and contains two calcium-binding motifs that regulate its conformational changes and interaction with myosin. This antibody aids analysis of MYL3's role in cardiac contractility and muscle development.

UniProt: P08590