PASK antibody (C-Term)

Catalog No. ABIN7877453

This Rabbit Polyclonal antibody specifically detects PASK in FACS. It exhibits reactivity toward Human.

Quick Overview for PASK antibody (C-Term) (ABIN7877453)

Target: PASK (PAS Domain Containing Serine/threonine Kinase (PASK))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PASK antibody is un-conjugated

Application: Flow Cytometry (FACS)

Product Details anti-PASK Antibody

Binding Specificity: C-Term

Purpose: PASK Antibody / PAS kinase

Purification: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human PASK was used as the immunogen for the PASK antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PASK antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the PASK antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PASK

Target: PASK (PAS Domain Containing Serine/threonine Kinase (PASK))

Alternative Name: PASK

Background: PASK antibody detects PAS domain-containing serine/threonine-protein kinase, an evolutionarily conserved enzyme that functions as a nutrient and energy sensor linking cellular metabolism with transcriptional control. Encoded by the PASK gene on chromosome 2q37.3, this kinase contains a C-terminal catalytic domain and N-terminal PAS (Per-Arnt-Sim) domains that sense changes in oxygen, redox potential, and small-molecule metabolites. PASK acts as a molecular integrator that coordinates energy homeostasis, glucose metabolism, and mitochondrial biogenesis in response to nutrient availability. The PAS domains enable regulation of its kinase activity through allosteric mechanisms, allowing PASK to respond dynamically to metabolic states.

PASK is broadly expressed in liver, skeletal muscle, pancreatic islets, and the brain, tissues that exhibit strong metabolic plasticity. In hepatocytes, PASK influences glycogen synthesis and gluconeogenic pathways, while in pancreatic beta cells it modulates insulin secretion. Mice lacking PASK exhibit altered glucose tolerance, reduced hepatic glycogen storage, and impaired mitochondrial function, demonstrating its role in metabolic adaptation. PASK phosphorylates transcriptional coactivators such as PGC-1alpha and CREB-regulated transcriptional coactivator 2 (CRTC2), linking nutrient signaling with gene expression that governs oxidative metabolism.

The PASK antibody is used in metabolic research to detect PASK expression and activation across tissues. Western blotting typically identifies a ~160 kilodalton band corresponding to the full-length kinase, while immunofluorescence localizes it to the cytoplasm and nucleus depending on cell type. In mammalian systems, PASK activity rises during fasting and decreases in nutrient-replete conditions, reflecting its regulatory role in energy balance. Because dysregulation of PASK signaling has been associated with diabetes, obesity, and mitochondrial dysfunction, this antibody provides an important tool for investigating metabolic signaling networks.

Recent studies also implicate PASK in circadian rhythm regulation and differentiation of muscle and stem cells. Its PAS domains act as environmental sensors, adjusting transcriptional programs to oxygen and redox changes. PASK interacts with AMPK and mTOR pathways, highlighting its integration into major metabolic signaling circuits. The PASK antibody enables researchers to explore these regulatory interactions and to characterize PASK as a potential therapeutic target in metabolic disease and aging. NSJ Bioreagents provides this antibody validated for its applications, ensuring precise detection across human and model systems.

UniProt: Q96RG2

Pathways: Regulation of Carbohydrate Metabolic Process