Recombinant ANO1 antibody (full length)

Catalog No. ABIN7877684

This Mouse Monoclonal antibody specifically detects ANO1 in IHC (p). It exhibits reactivity toward Human.

Quick Overview for Recombinant ANO1 antibody (full length) (ABIN7877684)

Target: ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ANO1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RDG1-8750

Product Details anti-ANO1 Antibody

Binding Specificity: full length

Purpose: Recombinant ANO1 Antibody / DOG1 / TMEM16A

Purification: Protein G affinity chromatography

Immunogen: A recombinant human full length protein was used as the immunogen for this recombinant ANO1 antibody.

Isotype: IgG2b, kappa

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant ANO1 antibody to be titered up or down for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant ANO1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for ANO1

Target: ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))

Alternative Name: ANO1

Background: Expression of DOG1/ANO1 protein is elevated in the gastrointestinal stromal tumors (GIST's), c-kit signaling-driven mesenchymal tumors of the GI tract. It is rarely expressed in other soft tissue tumors, which, due to appearance, may be difficult to diagnose. Immunoreactivity for ANO1 has been reported in 97.8 percent of scorable GIST's, including all c-kit negative GIST's. Overexpression has been suggested to aid in the identification of GISTs, including Platelet-Derived Growth Factor Receptor Alpha mutants that fail to express c-kit antigen. The overall sensitivity of ANO1 and c-kit in GIST's is nearly identical: 94.4 % vs. 94.7 %.

UniProt: Q5XXA6