Calpain antibody (full length)

Catalog No. ABIN7877760

The Mouse Monoclonal anti-Calpain antibody is suitable to detect Calpain in samples from Human. It has been validated for IHC (p).

Quick Overview for Calpain antibody (full length) (ABIN7877760)

Target: Calpain

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Calpain antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: CAPN1-1530

Product Details anti-Calpain Antibody

Binding Specificity: full length

Purpose: Calpain Antibody / CAPN1

Purification: Protein G affinity chromatography

Immunogen: A recombinant full-length human CAPN1 protein was used as the immunogen for this Calpain antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the Calpain antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the Calpain antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for Calpain

Target: Calpain

Background: Calpain 1 is an intracellular calcium-dependent protease that cleaves cytoskeletal and sub-membranous proteins. Calpains are non-lysosomal, calcium-activated intracellular cysteine proteases. Calpains mediate specific Ca2+- dependent processes including cell fusion, mitosis and meiosis. Calpains are heterodimers of a small regulatory subunit and one of three large catalytic subunits, designated Calpain 1, Calpain 2 and Calpain p94. Calpastatin regulates Calpain by inhibiting both the proteolytic activity of Calpain and its binding to membranes. Calpastatin exists in two types, tissue type and erythrocyte type, resulting from both alternative splicing and proteolytic processing. Calpain 1 co-localizes with human leukocyte antigen-DR (HLA-DR) on activated microglia in the aging brain. Calpain influences the process of spermatogenesis and the events preceding fertilization, such as the acrosome reaction.

UniProt: P07384