Recombinant Cyclin B1 antibody (full length)

Catalog No. ABIN7878061

The Rabbit Monoclonal anti-Cyclin B1 antibody (Clone CCNB1-7030R) (ABIN7878061) specifically detects Cyclin B1 in ELISA and IHC (p). The antibody is reactive with Human samples.

Quick Overview for Recombinant Cyclin B1 antibody (full length) (ABIN7878061)

Target: Cyclin B1 (CCNB1)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Cyclin B1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: CCNB1-7030R

Product Details anti-Cyclin B1 Antibody

Binding Specificity: full length

Purpose: Cyclin B1 Antibody (azide and preservative free)

Purification: Protein A/G affinity

Immunogen: Recombinant full-length human CCNB1 protein was used as the immunogen for the Cyclin B1 antibody.

Isotype: IgG, kappa

Application Details

Application Notes: Optimal dilution of the Cyclin B1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: -20 °C

Storage Comment: Aliquot the Cyclin B1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for Cyclin B1

Target: Cyclin B1 (CCNB1)

Alternative Name: Cyclin B1

Background: It recognizes a protein of 55-62 kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.

UniProt: P14635

Pathways: Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase