DNMT3A antibody (full length)

Catalog No. ABIN7878140

This Mouse Monoclonal antibody specifically detects DNMT3A in WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for DNMT3A antibody (full length) (ABIN7878140)

Target: DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This DNMT3A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: PCRP-DNMT3A-1E2

Product Details anti-DNMT3A Antibody

Binding Specificity: full length

Purpose: Dnmt3a Antibody (azide and preservative free)

Purification: Protein G affinity chromatography

Immunogen: Recombinant full length human protein was used as the immunogen for this Dnmt3a antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Dnmt3a antibody to be titered up or down for optimal performance.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Dnmt3a antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for DNMT3A

Target: DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))

Alternative Name: Dnmt3a

Background: DNMT3A is required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity.

UniProt: Q16655