DMC1 antibody (full length)

Catalog No. ABIN7878142

The Mouse Monoclonal anti-DMC1 antibody (Clone 2H12-4) (ABIN7878142) specifically detects DMC1 in WB, IF and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for DMC1 antibody (full length) (ABIN7878142)

Target: DMC1 (DNA Meiotic Recombinase 1 (DMC1))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This DMC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: 2H12-4

Product Details anti-DMC1 Antibody

Binding Specificity: full length

Purpose: DMC1 Antibody (azide and preservative free)

Purification: Protein G affinity chromatography

Immunogen: A recombinant full-length human protein was used as the immunogen for the DMC1 antibody.

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal dilution of the DMC1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the DMC1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for DMC1

Target: DMC1 (DNA Meiotic Recombinase 1 (DMC1))

Alternative Name: DMC1

Background: DNA repair proteins are necessary for the maintenance of chromosome integrity and are involved in the elimination of premutagenic lesions from DNA. The DNA repair proteins Rad51 and Rad52 are key components of the doublestrand-break repair (DSBR) pathway. Rad51 is essential for mitotic and meiotic recombination, and its mutation in yeast and mammalian cells results in chromosome loss. Overexpression of Rad52 confers resistance to ionizing radiation and induces homologous intrachromosomal recombination. Rad52 is thought to be involved in an early stage of Rad51-mediated recombination. Additional proteins involved in the pathway include Nibrin and Dmc1 (Meiotic recombination protein DMC1/LIM15 homolog). Nibrin, which complexes with Mre11 and Rad50, is absent in Nijemegen breakage syndrome (NBS) patients. Dmc1 is specifically involved in meiotic recombination. An alternative spliced form of Dmc1, designated Dmc1-D, is deleted for a region between the two motifs involved in nucleotide binding. The alternatively spliced Dmc1-D transcript is detected in both male and female germ cells, indicating that the encoded protein may have a role in mammalian genetic recombination in meiosis.

UniProt: Q14565