EIF2A antibody (full length)

Catalog No. ABIN7878206

This Mouse Monoclonal antibody specifically detects EIF2A in WB, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for EIF2A antibody (full length) (ABIN7878206)

Target: EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EIF2A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: PCRP-EIF2S1-1E2

Product Details anti-EIF2A Antibody

Binding Specificity: full length

Purpose: EIF2A Antibody

Purification: Protein A/G affinity

Immunogen: Recombinant full-length human EIF2S1 protein was used as the immunogen for the EIF2A antibody.

Isotype: IgG1

Application Details

Application Notes: Optimal dilution of the EIF2A antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the EIF2A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for EIF2A

Target: EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))

Alternative Name: EIF2A

Background: The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex is composed of three subunits, designated eIF2a, eIF2b and eIF2g (eukaryotic translation initiation factor 2 a, band g, respectively), all of which work in concert to form a ternary complex with GTP and tRNA in the early stages of protein synthesis. eIF2a, also known as EIF2S1 or EIF2, is a 315 amino acid subunit of the eukaryotic initiation complex that functions to bind tRNA to the 40S ribosomal subunit (in a GTP-dependent manner), thereby initiating translation. In addition, the phosphorylation state of eIF2a controls the rate of tRNA translation. When eIF2a is not phosphorylated, translation occurs at a normal rate. However, upon phosphorylation by one of several kinases, eIF2a is stabilized, thus preventing the GDP/GTP exchange reaction and slowing translation.

UniProt: P05198

Pathways: Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C, Methionine Biosynthetic Process, Ribosome Assembly