EIF2A antibody (full length)

Catalog No. ABIN7878206

This Mouse Monoclonal antibody specifically detects EIF2A in WB, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for EIF2A antibody (full length) (ABIN7878206)

Target: EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EIF2A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: PCRP-EIF2S1-1E2

Product Details anti-EIF2A Antibody

Binding Specificity: full length

Purpose: EIF2S1 Antibody

Purification: Protein A/G affinity

Immunogen: Recombinant full-length human EIF2S1 protein was used as the immunogen for the EIF2S1 antibody.

Isotype: IgG1

Application Details

Application Notes: Optimal dilution of the EIF2S1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles

Storage: -20 °C

Storage Comment: Aliquot the EIF2S1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for EIF2A

Target: EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))

Alternative Name: EIF2A

Background: Eukaryotic initiation factor 2 subunit 1 (EIF2S1), commonly known as eIF2 alpha, is a central regulator of translation initiation and cellular stress responses. EIF2S1 Antibody, clone PCRP-EIF2S1-1E2, is designed to detect this critical component of the heterotrimeric eIF2 complex, which delivers initiator methionyl-tRNA to the ribosome during the early stages of protein synthesis. EIF2S1 plays a key role in controlling global protein translation rates and serves as a major regulatory node linking environmental stress signals to translational control.

Under normal conditions, EIF2S1 forms a ternary complex with GTP and initiator tRNA, enabling efficient translation initiation at AUG start codons. However, in response to stress signals such as endoplasmic reticulum stress, oxidative stress, nutrient deprivation, or viral infection, EIF2S1 is phosphorylated at regulatory residues including Ser51. This phosphorylation inhibits the activity of the eIF2 complex, leading to a reduction in global protein synthesis while allowing selective translation of stress-response genes. This adaptive mechanism helps cells conserve resources and prioritize survival pathways.

EIF2S1 integrates signals from multiple upstream kinases, including PERK, PKR, GCN2, and HRI, each activated by distinct stress conditions. Through this network, EIF2S1 functions as a convergence point for diverse cellular stress pathways, coordinating translational repression and selective gene expression. Its regulation is essential for maintaining cellular homeostasis and responding to environmental challenges.

Subcellularly, EIF2S1 is predominantly localized in the cytoplasm, where it associates with ribosomes and translation initiation complexes. Immunofluorescence analysis typically reveals diffuse cytoplasmic staining with potential enrichment in perinuclear regions and ribonucleoprotein assemblies. Under stress conditions, EIF2S1 may be associated with stress granules and other dynamic structures involved in mRNA storage and translational control, reflecting shifts in protein synthesis activity.

In contrast to EIF2A, which supports alternative translation initiation pathways independent of the canonical eIF2 complex, EIF2S1 is a core component of the primary translation machinery and directly regulates global protein synthesis. Detection of EIF2S1 provides insight into baseline translational capacity, while changes in its phosphorylation state reflect activation of stress signaling pathways and translational repression mechanisms.

Dysregulation of EIF2S1 signaling has been implicated in cancer, neurodegenerative disorders, and metabolic diseases. Persistent activation of eIF2 alpha phosphorylation pathways can alter cell survival, apoptosis, and adaptive responses to stress. In tumor biology, modulation of EIF2S1 activity may support cancer cell survival under hypoxic or nutrient-limited conditions. These features support the use of an EIF2S1 Antibody in studies of translation control, stress signaling, and disease-associated changes in protein synthesis.

Microarray-based specificity validation demonstrates strong and selective binding of clone PCRP-EIF2S1-1E2 to EIF2S1, with a high Z-score and clear separation from non-target proteins. This supports reliable detection of eIF2 alpha in complex protein environments and reinforces its utility in applications such as immunofluorescence and flow cytometry for analysis of translational regulation and stress response pathways.

UniProt: P05198

Pathways: Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C, Methionine Biosynthetic Process, Ribosome Assembly