MITF antibody (full length)

Catalog No. ABIN7878618

The Mouse Monoclonal anti-MITF antibody is suitable to detect MITF in samples from Human. It has been validated for WB, IF, FACS and IP.

Quick Overview for MITF antibody (full length) (ABIN7878618)

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MITF antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: PCRP-MITF-1D9

Product Details anti-MITF Antibody

Binding Specificity: full length

Purpose: MITF Antibody

Purification: Protein A/G affinity

Immunogen: Recombinant full-length human MITF protein was used as the immunogen for the MITF antibody.

Isotype: IgG2b

Application Details

Application Notes: Optimal dilution of the MITF antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the MITF antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for MITF

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Alternative Name: MITF

Background: MITF (microphthalmia-associated transcription factor) is a melanocytic nuclear protein that contains basic helix-loop-helix (HLH) and leucine zipper (LZ) domains. These protein motifs are frequently observed in other transcription factors and are particularly common to members of the Myc family. MITF can directly associate with DNA as a homodimer and is required for the development and differentiation of melanocytes. Its expression is upregulated by cAMP and cAMP-dependent pathways. MITF activates several different gene promoters by binding to their E-boxes. Tyrosinase, TRP1 and TRP2 are pigment synthesis genes activated by MITF. When MITF is phosphorylated on Ser73 (via the MAPK pathway), it associates with co-activators of the p300/CBP family and enhances transcription. MITF has several isoforms including MITF-M which is specifically expressed in melanocytes. In MITFdeficient mice there is a complete absence of melanocytes.

UniProt: O75030

Pathways: Chromatin Binding