MLH1 antibody (full length)

Catalog No. ABIN7878664

The Mouse Monoclonal anti-MLH1 antibody (Clone MLH1-6467) (ABIN7878664) specifically detects MLH1 in IHC (p). The antibody is reactive with Human samples.

Quick Overview for MLH1 antibody (full length) (ABIN7878664)

Target: MLH1 (MutL Homolog 1 (MLH1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MLH1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: MLH1-6467

Product Details anti-MLH1 Antibody

Binding Specificity: full length

Purpose: MLH1 Antibody / MutL Homolog 1

Purification: Protein A/G affinity

Immunogen: Recombinant full-length human MLH1 protein was used as the immunogen for the MLH1 antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the MLH1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the MLH1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for MLH1

Target: MLH1 (MutL Homolog 1 (MLH1))

Alternative Name: MLH1

Background: This MAb recognizes a protein of 83 kDa, identified as MLH1. Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2 (HNPCC2). Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process, which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma, which plays a role in meiosis.

UniProt: P40692

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response