NMI antibody (full length)

Catalog No. ABIN7878700

The Mouse Monoclonal anti-NMI antibody (Clone PCRP-NMI-1C1) (ABIN7878700) specifically detects NMI in FACS. The antibody is reactive with Human samples.

Quick Overview for NMI antibody (full length) (ABIN7878700)

Target: NMI (N-Myc (And STAT) Interactor (NMI))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NMI antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: PCRP-NMI-1C1

Product Details anti-NMI Antibody

Binding Specificity: full length

Purpose: N-Myc Interactor Antibody / NMI / N-myc and STAT interactor

Purification: Protein A/G affinity

Immunogen: Recombinant full-length human N-Myc Interactor protein was used as the immunogen for the N-Myc Interactor antibody.

Isotype: IgG2b

Application Details

Application Notes: Optimal dilution of the N-Myc Interactor antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the N-Myc Interactor antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for NMI

Target: NMI (N-Myc (And STAT) Interactor (NMI))

Alternative Name: N-Myc Interactor

Background: Nmi (for N-Myc interactor) is an interferon inducible protein that associates with multiple transcription factors, including c-Myc, n-Myc, Max, and c-Fos, which contain bHLH-ZIP, bHLH, or Zip domains. Nmi is ubiquitously expressed at low levels throughout various fetal and adult tissues and at higher levels in myeloid leukemias and cell lines overexpressing c-Myc. In addition to binding Myc proteins, Nmi also associates with the Stat family of transcription factors, where it enhances Stat-dependent transcription. Although Nmi lacks an intrinsic DNA binding or activation domain, Nmi enhances the transcriptional activity of the Stat proteins, in response to cytokine stimulation, by recruiting the Stat1 and Stat5 transcriptional coactivators, CREB-binding protein (CBP) and p300. In vitro studies indicate that Nmi, expressed in conjunction with CBP, enhances the transcriptional responsiveness of Stat5 to IL-2 stimulation five-fold over CBP alone by increasing the affinity of Stat proteins for CBP/p300.

UniProt: Q13287