NRF1 antibody (full length)

Catalog No. ABIN7878725

This Mouse Monoclonal antibody specifically detects NRF1 in WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for NRF1 antibody (full length) (ABIN7878725)

Target: NRF1 (Nuclear Respiratory Factor 1 (NRF1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NRF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: NRF1-2609

Product Details anti-NRF1 Antibody

Binding Specificity: full length

Purpose: NRF1 Antibody / Nuclear Respiratory Factor 1

Purification: Protein G affinity chromatography

Immunogen: A recombinant full-length human protein was used as the immunogen for this NRF1 antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the NRF1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the NRF1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for NRF1

Target: NRF1 (Nuclear Respiratory Factor 1 (NRF1))

Alternative Name: NRF1

Background: The NF-E2 DNA binding protein is composed of two subunits, p45 and MafK, and it regulates expression of globin genes in developing erythroid cells through interaction with Maf recognition elements (MAREs). A family of NF-E2 related proteins, which are collectively known as the Cap 'n' collar (CNC) family and include Nrf1 (also designated TCF11), Nrf2 and Nrf3, are bZIP transcription factors that heterodimerize with Maf proteins to bind MARE sequences. The Nrf proteins also bind the antioxidant response element (ARE) and are implicated in the regulation of detoxification enzymes and the oxidative stress response. They do so by heterodimerizing with Jun family members (c-Jun, JunB and JunD) to activate gene expression, specifically the detoxifying enzyme, NQO1. Nrf2 is widely expressed and is thought to translocate to the nucleus after treatment with xenobiotics and antioxidants, which stimulate its release from a repressor protein Keap1. Nrf3 is highly expressed in placenta, B cells and monocytes.

UniProt: Q16656

Pathways: Regulation of Lipid Metabolism by PPARalpha