Recombinant Nucleolin antibody (full length)

Catalog No. ABIN7878735

The Rabbit Monoclonal anti-Nucleolin antibody is suitable to detect Nucleolin in samples from Human. It has been validated for WB, IF, FACS and IHC (p).

Quick Overview for Recombinant Nucleolin antibody (full length) (ABIN7878735)

Target: Nucleolin (NCL)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Nucleolin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: NCL-7014R

Product Details anti-Nucleolin Antibody

Binding Specificity: full length

Purpose: Nucleolin Antibody

Purification: Protein A affinity chromatography

Immunogen: Recombinant full length human protein was used as the immunogen for the Nucleolin antibody.

Isotype: IgG, kappa

Application Details

Application Notes: Optimal dilution of the Nucleolin antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the Nucleolin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for Nucleolin

Target: Nucleolin (NCL)

Alternative Name: Nucleolin

Background: Recognizes a protein of ~76 kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells.

UniProt: P19338

Pathways: Ribonucleoprotein Complex Subunit Organization