Recombinant Nucleolin antibody (full length)

Catalog No. ABIN7878738

The Rabbit Monoclonal anti-Nucleolin antibody is suitable to detect Nucleolin in samples from Human. It has been validated for WB and IHC (p).

Quick Overview for Recombinant Nucleolin antibody (full length) (ABIN7878738)

Target: Nucleolin (NCL)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Nucleolin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: NCL-8695R

Product Details anti-Nucleolin Antibody

Binding Specificity: full length

Purpose: Nucleolin Antibody / NCL (azide and preservative free)

Purification: Protein A/G affinity

Immunogen: Recombinant full-length human NCL protein was used as the immunogen for the Nucleolin antibody.

Isotype: IgG, kappa

Application Details

Application Notes: Optimal dilution of the Nucleolin antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: -20 °C

Storage Comment: Aliquot the Nucleolin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for Nucleolin

Target: Nucleolin (NCL)

Alternative Name: Nucleolin

Background: Recognizes a protein of ~76 kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This mAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

UniProt: P19338

Pathways: Ribonucleoprotein Complex Subunit Organization