ORC1 antibody (full length)

Catalog No. ABIN7878763

The Mouse Monoclonal anti-ORC1 antibody (Clone 7F6-1) (ABIN7878763) specifically detects ORC1 in ELISA. The antibody is reactive with Human samples.

Quick Overview for ORC1 antibody (full length) (ABIN7878763)

Target: ORC1 (ORC1L) (Origin Recognition Complex, Subunit 1 (ORC1L))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ORC1 antibody is un-conjugated

Application: ELISA

Clone: 7F6-1

Product Details anti-ORC1 Antibody

Binding Specificity: full length

Purpose: ORC1 Antibody / Origin Recognition Complex 1

Purification: Protein G affinity chromatography

Immunogen: A recombinant full-length human ORC1 protein was used as the immunogen for this ORC1 antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the ORC1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the ORC1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for ORC1

Target: ORC1 (ORC1L) (Origin Recognition Complex, Subunit 1 (ORC1L))

Alternative Name: ORC1

Background: The initiation of DNA replication is a multi-step process that depends on the formation of pre-replication complexes, which trigger initiation. Among the proteins required for establishing these complexes are the origin recognition complex (ORC) proteins. ORC proteins bind specifically to origins of replication where they serve as scaffold for the assembly of additional initiation factors. Human ORC subunits 1-6 are expressed in the nucleus of proliferating cells and tissues, such as the testis. ORC1 and ORC2 are both expressed at equivalent concentrations throughout the cell cycle, however, only ORC2 remains stably bound to chromatin. ORC4 and ORC6 are also expressed constantly throughout the cell cycle. ORC2, ORC3, ORC4 and ORC5 form a core complex upon which ORC6 and ORC1 assemble. The formation of this core complex suggests that ORC proteins play a crucial role in the G1-S transition in mammalian cells.

UniProt: Q13415

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA