Recombinant Melanoma gp100 antibody (full length)

Catalog No. ABIN7878840

The Mouse Monoclonal anti-Melanoma gp100 antibody is suitable to detect Melanoma gp100 in samples from Human. It has been validated for IHC (p).

Quick Overview for Recombinant Melanoma gp100 antibody (full length) (ABIN7878840)

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Melanoma gp100 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RPMEL17-6821

Product Details anti-Melanoma gp100 Antibody

Binding Specificity: full length

Purpose: Recombinant PMEL17 Antibody / Melanoma gp100

Purification: Protein A/G affinity

Immunogen: Recombinant full-length human protein was used as the immunogen for the recombinant PMEL17 antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the recombinant PMEL17 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the recombinant PMEL17 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for Melanoma gp100

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Alternative Name: PMEL17

Background: The gp100 Molecule is a 100 kDa glycosylated protein that is cleaved into a small (26 kDa) carboxy-terminal fragment and a larger amino- terminal section (60 64 kDa), which is subsequently cleaved to generate 26 kDa and 34 38 kDa fragments. By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. This MAb stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. This MAb also stains Angiomyolipoma (PEComa).

UniProt: P40967